Technologies and Computational Analysis Strategies for CRISPR Applications

被引：30

作者：

Clement, Kendell ^{[1
,2
,3
]}

Hsu, Jonathan Y. ^{[1
,2
,3
]}

Canver, Matthew C. ^{[1
,2
,3
,4
]}

Joung, J. Keith ^{[1
,2
]}

Pinello, Luca ^{[1
,2
,3
]}

机构：

[1] Massachusetts Gen Hosp, Ctr Canc Res, Mol Pathol Unit, Charlestown, MA 02129 USA

[2] Harvard Med Sch, Dept Pathol, Boston, MA 02115 USA

[3] Broad Inst MIT & Harvard, Cambridge, MA 02142 USA

[4] New York Presbyterian Hosp, Weill Cornell Med, Dept Pathol & Lab Med, New York, NY USA

来源：

MOLECULAR CELL | 2020年 / 79卷 / 01期

关键词：

OFF-TARGET CLEAVAGE; DOUBLE-STRANDED BREAKS; REGULATORY ELEMENTS; GENETIC SCREENS; FUNCTIONAL GENOMICS; UNBIASED DETECTION; MAMMALIAN-CELLS; READ ALIGNMENT; POOLED SCREENS; GUIDE RNA;

D O I：

10.1016/j.molcel.2020.06.012

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The CRISPR-Cas system offers a programmable platform for eukaryotic genome and epigenome editing. The ability to perform targeted genetic and epigenetic perturbations enables researchers to perform a variety of tasks, ranging from investigating questions in basic biology to potentially developing novel therapeutics for the treatment of disease. While CRISPR systems have been engineered to target DNA and RNA with increased precision, efficiency, and flexibility, assays to identify off-target editing are becoming more comprehensive and sensitive. Furthermore, techniques to perform high-throughput genome and epigenome editing can be paired with a variety of readouts and are uncovering important cellular functions and mechanisms. These technological advances drive and are driven by accompanying computational approaches. Here, we briefly present available CRISPR technologies and review key computational advances and considerations for various CRISPR applications. In particular, we focus on the analysis of on- and off-target editing and CRISPR pooled screen data.

引用

页码：11 / 29

页数：19

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