Molecular spectroscopic studies on the interaction of morin with bovine serum albumin

被引:74
作者
Hu, Yan-Jun [1 ]
Yue, Hua-Li [1 ]
Li, Xiao-Ling [1 ]
Zhang, Si-Si [1 ]
Tang, E. [1 ]
Zhang, Li-Ping [1 ]
机构
[1] Hubei Normal Univ, Hubei Key Lab Pollutant Anal & Reuse Technol, Dept Chem, Huangshi 435002, Peoples R China
基金
中国国家自然科学基金;
关键词
Morin; Bovine serum albumin; Conformational investigation; Binding parameters; Structure-affinity relationship; DRUG BINDING-SITES; FLUORESCENCE; FLAVONOIDS;
D O I
10.1016/j.jphotobiol.2012.04.001
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The interaction between morin and bovine serum albumin (BSA) was studied using molecular spectroscopic approach at different temperatures under imitated physiological conditions. Quenching of intrinsic tryptophanyl fluorescence of BSA with increasing morin concentration is the actuating tool in the analysis. The obtained quenching mechanisms, binding constants, binding sites and corresponding thermodynamic parameters at different temperatures indicate that the hydrophobic interaction play a major role in the morin-BSA association. Binding affinity between morin and BSA was determined using Scatchard equation and the modified Stern-Volmer equation, and the corresponding Structure-affinity relationships of flavonoids were discussed. Site marker competitive displacement experiments demonstrated that morin binds with high affinity to site II (subdomain IIIA) of BSA. Furthermore, the circular dichroism spectral results indicated that the conformation of BSA changed in the presence of morin. In addition, the effect of some common metal ions on the binding constant between morin and BSA was examined. (c) 2012 Elsevier B.V. All rights reserved.
引用
收藏
页码:16 / 22
页数:7
相关论文
共 43 条
[1]   Stereo-Selectivity of Human Serum Albumin to Enantiomeric and Isoelectronic Pollutants Dissected by Spectroscopy, Calorimetry and Bioinformatics [J].
Ahmad, Ejaz ;
Rabbani, Gulam ;
Zaidi, Nida ;
Singh, Saurabh ;
Rehan, Mohd ;
Khan, Mohd Moin ;
Rahman, Shah Kamranur ;
Quadri, Zainuddin ;
Shadab, Mohd. ;
Ashraf, Mohd Tashfeen ;
Subbarao, Naidu ;
Bhat, Rajiv ;
Khan, Rizwan Hasan .
PLOS ONE, 2011, 6 (11)
[2]   Structural Stability as a Probe for Molecular Evolution of Homologous Albumins Studied by Spectroscopy and Bioinformatics [J].
Ahmad, Ejaz ;
Sen, Priyankar ;
Khan, Rizwan Hasan .
CELL BIOCHEMISTRY AND BIOPHYSICS, 2011, 61 (02) :313-325
[3]   Effect of Ionic Liquids on the Solution Structure of Human Serum Albumin [J].
Akdogan, Yasar ;
Junk, Matthias J. N. ;
Hinderberger, Dariush .
BIOMACROMOLECULES, 2011, 12 (04) :1072-1079
[4]   In Situ Transient Spectroscopy for the Study of Glucuronidase Activity within Serum Albumin [J].
Bueno, Carlos J. ;
Jimenez, M. Consuelo ;
Miranda, Miguel A. .
JOURNAL OF PHYSICAL CHEMISTRY B, 2009, 113 (19) :6861-6865
[5]   PRELIMINARY CRYSTALLOGRAPHIC STUDIES OF 4 CRYSTAL FORMS OF SERUM-ALBUMIN [J].
CARTER, DC ;
CHANG, B ;
HO, JX ;
KEELING, K ;
KRISHNASAMI, Z .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1994, 226 (03) :1049-1052
[6]   Morin sulfates/glucuronides exert anti-inflammatory activity on activated macrophages and decreased the incidence of septic shock [J].
Fang, SH ;
Hou, YC ;
Chang, WC ;
Hsiu, SL ;
Chao, PDL ;
Chiang, BL .
LIFE SCIENCES, 2003, 74 (06) :743-756
[7]   Structure-activity relationship of flavonoids derived from medicinal plants in preventing methylmercury-induced mitochondrial dysfunction [J].
Franco, Jeferson L. ;
Posser, Thais ;
Missau, Fabiana ;
Pizzolatti, Moacir G. ;
Santos, Adair R. S. ;
Souza, Diogo O. ;
Aschner, Michael ;
Rocha, Joao B. T. ;
Dafre, Alcir L. ;
Farina, Marcelo .
ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY, 2010, 30 (03) :272-278
[8]   In vitro assessment of the antiviral potential of trans-cinnamic acid, quercetin and morin against equid herpesvirus 1 [J].
Gravina, H. D. ;
Tafuri, N. F. ;
Silva Junior, A. ;
Fietto, J. L. R. ;
Oliveira, T. T. ;
Diaz, M. A. N. ;
Almeida, M. R. .
RESEARCH IN VETERINARY SCIENCE, 2011, 91 (03) :E158-E162
[9]   ATOMIC-STRUCTURE AND CHEMISTRY OF HUMAN SERUM-ALBUMIN [J].
HE, XM ;
CARTER, DC .
NATURE, 1992, 358 (6383) :209-215
[10]   ADMET - turning chemicals into drugs [J].
Hodgson, J .
NATURE BIOTECHNOLOGY, 2001, 19 (08) :722-726