Hoechst-tagged Fluorescein Diacetate for the Fluorescence Imaging-based Assessment of Stomatal Dynamics in Arabidopsis thaliana

被引：3

作者：

Takaoka, Yousuke ^{[1
,2
]}

Miyagawa, Saki ^{[3
]}

Nakamura, Akinobu ^{[4
]}

Egoshi, Syusuke ^{[1
]}

Tsukiji, Shinya ^{[4
,5
,6
]}

Ueda, Minoru ^{[1
,3
]}

机构：

[1] Tohoku Univ, Grad Sch Sci, Dept Chem, Sendai, Miyagi 9808578, Japan

[2] Japan Sci & Technol Agcy, Precursory Res Embryon Sci & Technol PREST, Chiyoda Ku, 5 Sanbancho, Tokyo 1020075, Japan

[3] Tohoku Univ, Grad Sch Life Sci, Dept Mol & Chem Life Sci, Sendai, Miyagi 9808578, Japan

[4] Nagoya Inst Technol, Dept Life Sci & Appl Chem, Showa Ku, Gokiso Cho, Nagoya, Aichi 4668555, Japan

[5] Nagoya Inst Technol, Dept Nanopharmaceut Sci, Showa Ku, Gokiso Cho, Nagoya, Aichi 4668555, Japan

[6] Nagoya Inst Technol, Frontier Res Inst Mat Sci FRIMS, Showa Ku, Gokiso Cho, Nagoya, Aichi 4668555, Japan

来源：

SCIENTIFIC REPORTS | 2020年 / 10卷 / 01期

关键词：

ABSCISIC-ACID; GUARD-CELL; K+ CHANNELS; FUSICOCCIN; RECEPTORS; RESPONSES; IMMUNITY; CLOSURE; PROBES; DNA;

D O I：

10.1038/s41598-020-62239-w

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

In plants, stomata regulate water loss through transpiration for plant growth and survival in response to various environmental stressors; and simple methods to assess stomatal dynamics are needed for physiological studies. Herein, we report a fluorescence-imaging-based method using fluorescein diacetate tagged with Hoechst 33342, a nuclear staining chemical probe (HoeAc(2)Fl) for the qualitative assessment of stomatal dynamics. In our method, the stomatal movement is inferred by simple monitoring of the fluorescence intensity in the nucleus of the stomata.

引用

页数：7