IN VITRO ANTIOXIDANT, HEMOLYTIC, THROMBOLYTIC POTENCIES OF Centratherum anthelminticum SEED EXTRACTSAND IT'S IN OVO ANTIVIRAL EFFICACY

Herbal medicines have always been in practice since ancient times due to their ease and efficiency. Therefore, methanolic, acetonic, chloroform and n-hexane extract from the seeds of Centratherum anthelminticum were prepared by soxhlet extraction method. These extracts were subjected to quantify antioxidant abilities like TPC, TFC, DPPH free radical scavenging, reducing power, radical scavenging capacity for hydroxyl and nitric oxide. The toxicity of the extract was assessed by hemolytic assay in vitro on human and poultry erythrocyte. However, the percentage clot lysis behaviour of these extracts was also analyzed by thrombolytic activity by using Clopidogrel as standard. Further, Antiviral effect was inspected by in ovo analysis against Newcastle disease virus (NDV). Seven groups of nine-day-old embryonic chicken eggs were inoculated with different concentrations of extracts. Except for the negative control group; all groups were inoculated with NDV strain and different concentrations of four extracts. Observed the embryo's survival every day. Hemagglutination was done by collecting allantoic fluid from experimental eggs. Results indicated that all the extracts have antioxidant potential and display the highest antiradical behaviour in the pattern of methanolic > acetonic > chloroform > n-hexane. The hemolytic activity showed that all extracts of C. anthelminticum are non/less toxic towards Human and poultry RBCs (< 15%). The amount of clot lysis ranged from 19 to 51% compared to 65% and 6.4%, respectively provide by positive control and negative control. In the case of the methanol extract, the level of clot lysis was the most abundant (51%). In the case of in ovo antiviral assay, the highest survival rate was observed in the methanolic extract at 400 mu g/mL and acetonic extract at 300 mu g/mL as they control the NDV activity ultimately, evidenced by the absence of embryo death and HA titre. Chloroform and n-hexane could not inhibit the virus completely.