Somatostatin Decorated Quantum Dots for Targeting of Somatostatin Receptors

被引:1
作者
Abdellatif, Ahmed Abdelfattah Hafez [1 ]
Abdelhafez, Wael Abdellah [1 ]
Sarhan, Hatem Abdelmunsef [2 ]
机构
[1] Al Azhar Univ, Fac Pharm, Dept Pharmaceut & Ind Pharm, Assiut, Egypt
[2] Menia Univ, Fac Pharm, Dept Pharmaceut & Ind Pharm, El Minia, Egypt
来源
IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH | 2018年 / 17卷 / 02期
关键词
Quantum dots; Somatostatin receptors; Somatostatin; Cellular uptake; Receptor targeting; IN-VIVO; REAGENT; DELIVERY; CELLS; NANOPARTICLES; EXPRESSION; DIAGNOSIS; INJECTION; PEPTIDES; ANALOGS;
D O I
暂无
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
Due to the unique optical properties like high brightness and narrow emission bands of Quantum dots, it is used as simple fluorescence materials in bio-imaging, immunoassays, microarrays, and other applications. To easy invistigate cell lines that overexpressed somtostatin receptors, somatostatin (SST) was conjugated with Quantum dots carrying PEG amine (Qdots-PEG-NH2). The conjugation of SST to Qdots-PEG-NH2 started with the thiolation of SST using Traut's reagent. Moreover, the Qdots-PEG-NH2 were subsequently activated by 500-fold molar excess of sulfosuccinimidyl 4-(N-maleimidomethyl) cyclohexane-1-carboxylate (sulfo-SMCC) dissolved in phosphate buffer. The Qdots-PEG-NH2-sulfo-SMCC was conjugated to the thiolated-SST to form Qdots-SST. The number of sulfhydryl groups can be controlled by the molar ratio of Traut's reagent to SST. Thiolation was necessary for the conjugation of SST to Qdots-PEG-NH2. This was achieved by reacting the SST with Traut's reagent in a 1: 1 molar ratio. Ellman's reagent was used to determine the number of sulfhydryle groups. Furthermore, cellular uptake study on triple negative breast cancer cells (HCC-1806) showed that the numbers of Qdots-SST per cell were significantly higher compared to unmodified Qdots-PEG-NH2 when quantified using inductively coupled plasma optical emission spectroscopy (ICPOES). Moreover, the binding of Qdots-SST to cells can be suppressed by addition of free SST, indicating that the binding of Qdots-SST to cells is due to receptor-specific binding.
引用
收藏
页码:513 / 524
页数:12
相关论文
共 50 条
[41]   The puzzle of somatostatin: Action, receptors, analogues and therapy [J].
Panteris, V ;
Karamanolis, DG .
HEPATO-GASTROENTEROLOGY, 2005, 52 (66) :1771-1781
[42]   Comparative distribution of somatostatin and somatostatin receptors in PTU-induced hypothyroidism [J].
Singh, Sneha ;
Somvanshi, Rishi K. ;
Panda, Vandana ;
Kumar, Ujendra .
ENDOCRINE, 2020, 70 (01) :92-106
[43]   Genetic abnormalities of somatostatin receptors in pituitary tumors [J].
Lania, A. ;
Mantovani, G. ;
Spada, A. .
MOLECULAR AND CELLULAR ENDOCRINOLOGY, 2008, 286 (1-2) :180-186
[44]   Somatostatin receptors and signaling cascades coupled to them [J].
Shpakov, A. O. .
JOURNAL OF EVOLUTIONARY BIOCHEMISTRY AND PHYSIOLOGY, 2012, 48 (04) :385-400
[45]   New somatostatin-drug conjugates for effective targeting pancreatic cancer [J].
Ragozin, E. ;
Hesin, A. ;
Bazylevich, A. ;
Tuchinsky, H. ;
Bovina, A. ;
Zahavi, T. Shekhter ;
Oron-Herman, M. ;
Kostenich, G. ;
Firer, M. A. ;
Rubinek, T. ;
Wolf, I. ;
Luboshits, G. ;
Sherman, M. Y. ;
Gellerman, G. .
BIOORGANIC & MEDICINAL CHEMISTRY, 2018, 26 (13) :3825-3836
[46]   Immunohistochemical localization and quantitative expression of somatostatin receptors in normal human spleen and thymus: Implications for the in vivo visualization during somatostatin receptor scintigraphy [J].
Ferone, D. ;
Pivonello, R. ;
Kwekkeboom, D. J. ;
Gatto, F. ;
Ameri, P. ;
Colao, A. ;
de Krijger, R. R. ;
Minuto, F. ;
Lamberts, S. W. J. ;
van Hagen, P. M. ;
Hofland, L. J. .
JOURNAL OF ENDOCRINOLOGICAL INVESTIGATION, 2012, 35 (05) :528-534
[47]   Ultraviolet A radiation induces cortistatin overexpression and activation of somatostatin receptors in ARPE-19 cells [J].
Clementi, Maria Elisabetta ;
Sampaolese, Beatrice ;
Lazzarino, Giacomo ;
Tringali, Giuseppe .
MOLECULAR MEDICINE REPORTS, 2018, 17 (04) :5538-5543
[48]   A Novel Lanreotide-Encoded Micelle System Targets Paclitaxel to the Tumors with Overexpression of Somatostatin Receptors [J].
Zheng, Nan ;
Dai, Wenbing ;
Du, Wenwen ;
Zhang, Haoran ;
Lei, Liandi ;
Zhang, Hua ;
Wang, Xueqing ;
Wang, Jiancheng ;
Zhang, Xuan ;
Gao, Jinming ;
Zhang, Qiang .
MOLECULAR PHARMACEUTICS, 2012, 9 (05) :1175-1188
[49]   Identification of somatostatin receptors using labeled PEGylated octreotide, as an active internalization [J].
Abdellatif, Ahmed A. H. .
DRUG DEVELOPMENT AND INDUSTRIAL PHARMACY, 2019, 45 (10) :1707-1715
[50]   Somatostatin receptors in pituitary and development of somatostatin receptor subtype-selective analogs [J].
Ilan Shimon .
Endocrine, 2003, 20 :265-269