Functional MR microimaging of pancreatic β-cell activation

被引:51
作者
Gimi, Barjor
Leoni, Lara
Oberholzer, Jose
Braun, Mark
Avila, Jose
Wang, Yong
Desai, Tejal
Philipson, Louis H.
Magin, Richard L.
Roman, Brian B.
机构
[1] Univ Chicago, Dept Radiol, Chicago, IL 60637 USA
[2] Univ Chicago, Dept Med, Sect Endorcinol, Chicago, IL 60637 USA
[3] Univ Illinois, Cardiovasc Res Ctr, Dept Physiol & Biophys, Chicago, IL 60612 USA
[4] Boston Univ, Dept Biomed Engn, Boston, MA USA
[5] Hosp Univ Illinois Chicago, Div Transplantat, Chicago, IL 60612 USA
[6] Univ Illinois, Dept Bioengn, Chicago, IL 60607 USA
[7] Johns Hopkins Univ, Sch Med, Russell H Morgan Dept Radiol & Radiol Sci, Baltimore, MD 21205 USA
关键词
MRI; islets; beta-cells; manganese; activation;
D O I
10.3727/000000006783982151
中图分类号
Q813 [细胞工程];
学科分类号
摘要
The increasing incidence of diabetes and the need to further understand its cellular basis has resulted in the development of new diagnostic and therapeutic techniques. Nonetheless, the quest to noninvasively ascertain P-cell mass and function has not been achieved. Manganese (Mn)-enhanced MRI is presented here as a tool to image P-cell functionality in cell culture and isolated islets. Similar to calcium, extracellular Mn was taken up by glucose-activated beta-cells resulting in 200% increase in MRI contrast enhancement, versus nonactivated cells. Similarly, glucose-activated islets showed an increase in MRI contrast up to 45%. Although glucose-stimulated Ca influx was depressed in the presence of 100 mu M Mn. no significant effect was seen at lower Mn concentrations. Moreover. islets exposed to Mn showed normal glucose sensitivity and insulin secretion. These results demonstrate a link between image contrast enhancement and beta-cell activation in vitro. and provide the basis for future noninvasive in vivo imaging of islet functionality and beta-cell mass.
引用
收藏
页码:195 / 203
页数:9
相关论文
共 45 条
[1]  
ANGTUACO TL, 1986, PHYSIOL CHEM PHYS M, V18, P41
[2]   ELECTROPHYSIOLOGY OF THE PANCREATIC BETA-CELL [J].
ASHCROFT, FM ;
RORSMAN, P .
PROGRESS IN BIOPHYSICS & MOLECULAR BIOLOGY, 1989, 54 (02) :87-143
[3]   Improvement of pancreatic islet isolation outcomes using glutamine perfusion during isolation procedure [J].
Avila, JG ;
Tsujimura, T ;
Oberholzer, J ;
Churchill, T ;
Salehi, P ;
Shapiro, AMJ ;
Lakey, JRT .
CELL TRANSPLANTATION, 2003, 12 (08) :877-881
[4]   NMR AND ESR STUDY OF LIPOSOME DELIVERY OF MN-2+ TO MURINE LIVER [J].
BACIC, G ;
NIESMAN, MR ;
MAGIN, RL ;
SWARTZ, HM .
MAGNETIC RESONANCE IN MEDICINE, 1990, 13 (01) :44-61
[5]   Effects of MnDPDP, DPDP--, and MnCl2 on cardiac energy metabolism and manganese accumulation - An experimental study in the isolated perfused rat heart [J].
Brurok, H ;
Schjott, J ;
Berg, K ;
Karlsson, JOG ;
Jynge, P .
INVESTIGATIVE RADIOLOGY, 1997, 32 (04) :205-211
[6]  
Cao DR, 1997, J CELL SCI, V110, P497
[7]   Detection of single mammalian cells by high-resolution magnetic resonance imaging [J].
Dodd, SJ ;
Williams, M ;
Suhan, JP ;
Williams, DS ;
Koretsky, AP ;
Ho, C .
BIOPHYSICAL JOURNAL, 1999, 76 (01) :103-109
[8]   Voltage-dependent entry and generation of slow Ca2+ oscillations in glucose-stimulated pancreatic β-cells [J].
Dryselius, S ;
Grapengiesser, E ;
Hellman, B ;
Gylfe, E .
AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM, 1999, 276 (03) :E512-E518
[9]   SIGNAL-TRANSDUCTION VIA P2-PURINERGIC RECEPTORS FOR EXTRACELLULAR ATP AND OTHER NUCLEOTIDES [J].
DUBYAK, GR ;
ELMOATASSIM, C .
AMERICAN JOURNAL OF PHYSIOLOGY, 1993, 265 (03) :C577-C606
[10]  
Dyachok O, 2001, J CELL SCI, V114, P2179