Microarray analyses reveal strain-specific antibody responses to Plasmodium falciparum apical membrane antigen 1 variants following natural infection and vaccination

被引：18

作者：

Bailey, Jason A. ^{[1
]}

Berry, Andrea A. ^{[1
]}

Travassos, Mark A. ^{[1
]}

Ouattara, Amed ^{[1
]}

Boudova, Sarah ^{[1
]}

Dotsey, Emmanuel Y. ^{[2
]}

Pike, Andrew ^{[1
]}

Jacob, Christopher G. ^{[3
]}

Adams, Matthew ^{[1
]}

Tan, John C. ^{[4
,5
]}

Bannen, Ryan M. ^{[4
,5
]}

Patel, Jigar J. ^{[4
,5
]}

Pablo, Jozelyn ^{[2
]}

Nakajima, Rie ^{[2
]}

Jasinskas, Algis ^{[2
]}

Dutta, Sheetij ^{[6
]}

Takala-Harrison, Shannon ^{[1
]}

Lyke, Kirsten E. ^{[1
]}

Laurens, Matthew B. ^{[1
]}

Niangaly, Amadou ^{[7
]}

Coulibaly, Drissa ^{[7
]}

Kouriba, Bourema ^{[7
]}

Doumbo, Ogobara K. ^{[7
]}

Thera, Mahamadou A. ^{[7
]}

Felgner, Philip L. ^{[2
]}

Plowe, Christopher, V ^{[1
,8
]}

机构：

[1] Univ Maryland, Sch Med, Ctr Vaccine Dev & Global Hlth, Baltimore, MD 21201 USA

[2] Univ Calif Irvine, Dept Physiol & Biophys, Irvine, CA 92717 USA

[3] Wellcome Sanger Inst, Hinxton, England

[4] Roche Sequencing Solut, Madison, WI USA

[5] Nimble Therapeut, Madison, WI USA

[6] US Mil, Malaria Vaccine Program, Walter Reed Army Inst Res, Silver Spring, MD USA

[7] Univ Sci Tech & Technol Bamako, Malaria Res & Training Ctr, Bamako, Mali

[8] Duke Univ, Duke Global Hlth Inst, Durham, NC 27708 USA

来源：

SCIENTIFIC REPORTS | 2020年 / 10卷 / 01期

关键词：

GENETIC DIVERSITY; IMMUNE-RESPONSE; MALARIA; INVASION; AMA1; SEROREACTIVITY; PROTECTION; CHILDREN; COMPLEX; BINDING;

D O I：

10.1038/s41598-020-60551-z

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Vaccines based on Plasmodium falciparum apical membrane antigen 1 (AMA1) have failed due to extensive polymorphism in AMA1. To assess the strain-specificity of antibody responses to malaria infection and AMA1 vaccination, we designed protein and peptide microarrays representing hundreds of unique AMA1 variants. Following clinical malaria episodes, children had short-lived, sequence-independent increases in average whole-protein seroreactivity, as well as strain-specific responses to peptides representing diverse epitopes. Vaccination resulted in dramatically increased seroreactivity to all 263 AMA1 whole-protein variants. High-density peptide analysis revealed that vaccinated children had increases in seroreactivity to four distinct epitopes that exceeded responses to natural infection. A single amino acid change was critical to seroreactivity to peptides in a region of AMA1 associated with strain-specific vaccine efficacy. Antibody measurements using whole antigens may be biased towards conserved, immunodominant epitopes. Peptide microarrays may help to identify immunogenic epitopes, define correlates of vaccine protection, and measure strain-specific vaccine-induced antibodies.

引用

页数：12

共 48 条

[1] Duration of naturally acquired antibody responses to blood-stage Plasmodium falciparum is age dependent and antigen specific [J].

Akpogheneta, Onome J. ;

Duah, Nancy O. ;

Tetteh, Kevin K. A. ;

Dunyo, Samuel ;

Lanar, David E. ;

Pinder, Margaret ;

Conway, David J. .

INFECTION AND IMMUNITY, 2008, 76 (04) :1748-1755

[2] Structure of AMA1 from Plasmodium falciparum reveals a clustering of polymorphisms that surround a conserved hydrophobic pocket [J].

Bai, T ;

Becker, M ;

Gupta, A ;

Strike, P ;

Murphy, VJ ;

Anders, RF ;

Batchelor, AH .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2005, 102 (36) :12736-12741

[3] Short Report: Seroreactivity to a Large Panel of Field-Derived Plasmodium falciparum Apical Membrane Antigen 1 and Merozoite Surface Protein 1 Variants Reflects Seasonal and Lifetime Acquired Responses to Malaria [J].

Bailey, Jason A. ;

Pablo, Jozelyn ;

Niangaly, Amadou ;

Travassos, Mark A. ;

Ouattara, Amed ;

Coulibaly, Drissa ;

Laurens, Matthew B. ;

Takala-Harrison, Shannon L. ;

Lyke, Kirsten E. ;

Skinner, Jeff ;

Berry, Andrea A. ;

Jasinskas, Algis ;

Nakajima-Sasaki, Rie ;

Kouriba, Bourema ;

Thera, Mahamadou A. ;

Felgner, Philip L. ;

Doumbo, Ogobara K. ;

Plowe, Christopher V. .

AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 2015, 92 (01) :9-12

[4] CONTROLLING THE FALSE DISCOVERY RATE - A PRACTICAL AND POWERFUL APPROACH TO MULTIPLE TESTING [J].

BENJAMINI, Y ;

HOCHBERG, Y .

JOURNAL OF THE ROYAL STATISTICAL SOCIETY SERIES B-STATISTICAL METHODOLOGY, 1995, 57 (01) :289-300

[5] Genetic polymorphism of merozoite surface protein-1 in Plasmodium falciparum isolates from patients with mild to severe malaria in Libreville, Gabon [J].

Bouyou-Akotet, Marielle Karine ;

M'Bondoukwe, Noe Patrick ;

Mawili-Mboumba, Denise Patricia .

PARASITE, 2015, 22

[6] Rhoptry neck protein RON2 forms a complex with microneme protein AMA1 in Plasmodium falciparum merozoites [J].

Cao, Jun ;

Kaneko, Osamu ;

Thongkukiatkul, Amporn ;

Tachibana, Mayumi ;

Otsuki, Hitoshi ;

Gao, Qi ;

Tsuboi, Takafumi ;

Torii, Motomi .

PARASITOLOGY INTERNATIONAL, 2009, 58 (01) :29-35

[7] The most polymorphic residue on Plasmodium falciparum apical membrane antigen 1 determines binding of an invasion-inhibitory antibody [J].

Coley, AM ;

Parisi, K ;

Masciantonio, R ;

Hoeck, J ;

Casey, JL ;

Murphy, VJ ;

Harris, KS ;

Batchelor, AH ;

Anders, RF ;

Foley, M .

INFECTION AND IMMUNITY, 2006, 74 (05) :2628-2636

[8] Structure of the malaria antigen AMA1 in complex with a growth-inhibitory antibody [J].

Coley, Andrew M. ;

Gupta, Aditi ;

Murphy, Vince J. ;

Bai, Tao ;

Kim, Hanna ;

Anders, Robin F. ;

Foley, Michael ;

Batchelor, Adrian H. .

PLOS PATHOGENS, 2007, 3 (09) :1308-1319

[9] Allele specificity of naturally acquired antibody responses against Plasmodium falciparum apical membrane antigen 1 [J].

Cortés, A ;

Mellombo, M ;

Masciantonio, R ;

Murphy, VJ ;

Reeder, JC ;

Anders, RF .

INFECTION AND IMMUNITY, 2005, 73 (01) :422-430

[10] Stable malaria incidence despite scaling up control strategies in a malaria vaccine-testing site in Mali [J].

Coulibaly, Drissa ;

Travassos, Mark A. ;

Kone, Abdoulaye K. ;

Tolo, Youssouf ;

Laurens, Matthew B. ;

Traore, Karim ;

Diarra, Issa ;

Niangaly, Amadou ;

Daou, Modibo ;

Dembele, Ahmadou ;

Sissoko, Mody ;

Guindo, Boureima ;

Douyon, Raymond ;

Guindo, Aldiouma ;

Kouriba, Bourema ;

Sissoko, Mahamadou S. ;

Sagara, Issaka ;

Plowe, Christopher V. ;

Doumbo, Ogobara K. ;

Thera, Mahamadou A. .

MALARIA JOURNAL, 2014, 13

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