An Orthotopic Murine Model of Human Prostate Cancer Metastasis

被引:23
作者
Pavese, Janet [1 ]
Ogden, Irene M. [1 ]
Bergan, Raymond C. [1 ,2 ,3 ]
机构
[1] Northwestern Univ, Dept Med, Evanston, IL 60208 USA
[2] Northwestern Univ, Robert H Lurie Canc Ctr, Evanston, IL 60208 USA
[3] Northwestern Univ, Ctr Mol Innovat & Drug Discovery, Evanston, IL 60208 USA
来源
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS | 2013年 / 79期
基金
美国国家卫生研究院;
关键词
Medicine; Issue; 79; Urogenital System; Male Urogenital Diseases; Surgical Procedures; Operative; Life Sciences (General); Prostate Cancer; Metastasis; Mouse Model; Drug Discovery; Molecular Biology; MICE; MOUSE; BONE;
D O I
10.3791/50873
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Our laboratory has developed a novel orthotopic implantation model of human prostate cancer (PCa). As PCa death is not due to the primary tumor, but rather the formation of distinct metastasis, the ability to effectively model this progression pre-clinically is of high value. In this model, cells are directly implanted into the ventral lobe of the prostate in Balb/c athymic mice, and allowed to progress for 4-6 weeks. At experiment termination, several distinct endpoints can be measured, such as size and molecular characterization of the primary tumor, the presence and quantification of circulating tumor cells in the blood and bone marrow, and formation of metastasis to the lung. In addition to a variety of endpoints, this model provides a picture of a cells ability to invade and escape the primary organ, enter and survive in the circulatory system, and implant and grow in a secondary site. This model has been used effectively to measure metastatic response to both changes in protein expression as well as to response to small molecule therapeutics, in a short turnaround time.
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页数:9
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