Arginine exposure alters ectonucleotidase activities and morphology of zebrafish larvae (Danio rerio)

被引：16

作者：

Capiotti, Katiucia Marques ^{[1
,3
]}

Fazenda, Lidiane ^{[1
,3
]}

Nazario, Luiza Reali ^{[1
,3
]}

Menezes, Fabiano Peres ^{[1
,3
]}

Kist, Luiza Wilges ^{[2
,3
]}

Bogo, Mauricio Reis ^{[2
,3
]}

Da Silva, Rosane Souza ^{[1
,3
]}

Wyse, Angela Terezinha ^{[4
]}

Bonan, Carla Denise ^{[1
,3
]}

机构：

[1] Pontificia Univ Catolica Rio Grande do Sul, Dept Biol Celular & Mol, Lab Neuroquim & Psicofarmacol, Fac Biociencias, Porto Alegre, RS, Brazil

[2] Pontificia Univ Catolica Rio Grande do Sul, Dept Biol Celular & Mol, Lab Biol Genom & Mol, Fac Biociencias, Porto Alegre, RS, Brazil

[3] INCT TM, BR-90035003 Porto Alegre, RS, Brazil

[4] Univ Fed Rio Grande do Sul, Inst Ciencias Basicas Saude, Dept Bioquim, BR-90035003 Porto Alegre, RS, Brazil

来源：

INTERNATIONAL JOURNAL OF DEVELOPMENTAL NEUROSCIENCE | 2013年 / 31卷 / 01期

关键词：

Arginine; NTPDase; Ecto-5 '-nucleotidase; Development; Hyperargininemia; Zebrafish; NUCLEOTIDE HYDROLYSIS; ADENOSINE RECEPTORS; BRAIN MEMBRANES; ATP; SYNAPTOSOMES; INHIBITION; BEHAVIOR; DISEASE; SYSTEM; CORTEX;

D O I：

10.1016/j.ijdevneu.2012.09.002

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Hyperargininemia is an inborn error of metabolism (IEM) characterized by tissue accumulation of arginine (Arg). Mental retardation and other neurological features are common symptoms in hyperargininemic patients. Considering purinergic signaling has a crucial role from the early stages of development and underlying mechanisms of this disease are poorly established, we investigated the effect of Arg administration on locomotor activity, morphological alterations, and extracellular nucleotide hydrolysis in larvae and adult zebrafish. We showed that 0.1 mM Arg was unable to promote changes in locomotor activity. In addition, 7-day-post-fertilization (dpf) larvae treated with Arg demonstrated a decreased body size. Arg exposure (0.1 mM) promoted an increase in ATP, ADP, and AMP hydrolysis when compared to control group. These findings demonstrated that Arg might affect morphological parameters and ectonucleotidase activities in zebrafish larvae, suggesting that purinergic system is a target for neurotoxic effects induced by Arg. (C) 2012 ISDN. Published by Elsevier Ltd. All rights reserved.

引用

页码：75 / 81

页数：7

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