Transcutaneous measurement of renal function in conscious mice

被引：174

作者：

Schreiber, Andrea ^{[3
]}

Shulhevich, Yury

Geraci, Stefania ^{[1
,2
,4
]}

Hesser, Juergen

Stsepankou, Dzmitry

Neudecker, Sabine ^{[1
,2
,4
]}

Koenig, Stefan ^{[5
]}

Heinrich, Ralf ^{[5
]}

Hoecklin, Friederike ^{[5
]}

Pill, Johannes ^{[6
,7
]}

Friedemann, Jochen ^{[1
,2
]}

Schweda, Frank ^{[3
]}

Gretz, Norbert ^{[1
,2
,4
]}

Schock-Kusch, Daniel ^{[1
,2
,4
]}

机构：

[1] Univ Heidelberg, Inst Med Technol, D-68167 Mannheim, Germany

[2] Univ Appl Sci, D-68167 Mannheim, Germany

[3] Univ Regensburg, Inst Physiol, Regensburg, Germany

[4] Univ Heidelberg, Med Fac Mannheim, Med Res Ctr, D-68167 Mannheim, Germany

[5] Freudenberg Forsch Dienste, Weinheim, Germany

[6] Mektec Europe, Weinheim, Germany

[7] Mannheim Pharma & Diagnost, Mannheim, Germany

来源：

AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY | 2012年 / 303卷 / 05期

关键词：

fluorescein isothiocyanate-sinistrin; kidney function; glomerular filtration rate; transcutaneous; fluorometry; GLOMERULAR-FILTRATION-RATE; FLUORESCEIN-LABELED SINISTRIN; FITC-SINISTRIN; MARKER; RATS; CLEARANCE; GFR;

D O I：

10.1152/ajprenal.00279.2012

中图分类号：

Q4 [生理学];

学科分类号：

071003 ;

摘要：

Schreiber A, Shulhevich Y, Geraci S, Hesser J, Stsepankou D, Neudecker S, Koenig S, Heinrich R, Hoecklin F, Pill J, Friedemann J, Schweda F, Gretz N, Schock-Kusch D. Transcutaneous measurement of renal function in conscious mice. Am J Physiol Renal Physiol 303: F783-F788, 2012. First published June 13, 2012; doi: 10.1152/ajprenal.00279.2012.-Determination of glomerular filtration rate (GFR) in conscious mice is cumbersome for the experimenter and stressful for the animals. Here we report on a simple new technique allowing the transcutaneous measurement of GFR in conscious mice. This approach extends our previously developed technique for rats to mice. The technique relies on a miniaturized device equipped with an internal memory that permits the transcutaneous measurement of the elimination kinetics of the fluorescent renal marker FITC-sinistrin. This device is described and validated compared with FITC-sinistrin plasma clearance in healthy, unilaterally nephrectomized and pcy mice. In summary, we describe a technique allowing the measurement of renal function in freely moving mice independent of blood or urine sampling as well as of laboratory assays.

引用

页码：F783 / F788

页数：6