An overview of applications of CRISPR-Cas technologies in biomedical engineering

被引:7
作者
Jamehdor, Saleh [1 ,2 ,3 ]
Zaboli, Kasra Arbabi [4 ]
Naserian, Sina [5 ,6 ,7 ]
Thekkiniath, Jose [8 ]
Omidy, Honey Alef [9 ]
Teimoori, Ali [1 ]
Johari, Behrooz [4 ]
Taromchi, Amir Hossein [4 ]
Sasano, Yu [10 ]
Kaboli, Saeed [4 ]
机构
[1] Hamadan Univ Med Sci, Fac Med, Dept Virol, Hamadan, Hamadan, Iran
[2] Natl Inst Genet Engn & Biotechnol, Inst Med Biotechnol, Dept Mol Med, Tehran, Iran
[3] Univ Sistan & Baluchestan, Fac Sci, Dept Biol, Zahedan, Iran
[4] Zanjan Univ Med Sci, Sch Med, Dept Med Biotechnol, Zanjan, Iran
[5] Paul Brousse Hosp Villejuif, INSERM UMR S MD 1197, Minist Armed Forces, Biomed Res Inst Armed Forces IRBA, Villejuif, France
[6] CTSA Clamart, Clamart, France
[7] Sivan Aryo Pharmed, SivanCell, Tehran, Iran
[8] Fuller Labs, Fullerton, CA USA
[9] Univ Southern Calif, Dept Biochem & Mol Biol, Los Angeles, CA 90007 USA
[10] Sojo Univ, Fac Biotechnol & Life Sci, Dept Appl Microbial Technol, Kumamoto, Japan
关键词
Genome editing; sgRNA; CRISPR-Cas9; CRISPR-Cas12; CRISPR-Cas13; GUIDE RNA; MYCOBACTERIUM-TUBERCULOSIS; CRYSTAL-STRUCTURE; ESCHERICHIA-COLI; KNOCK-IN; GENOME; GENE; DNA; TRANSCRIPTION; ACTIVATION;
D O I
10.5603/FHC.a2020.0023
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Clustered Regulatory Interspaced Short Palindromic Repeats (CRISPR) is one of the major genome editing systems and allows changing DNA levels of an organism. Among several CRISPR categories, the CRISPR-Cas9 system has shown a remarkable progression rate over its lifetime. Recently, other tools including CRISPR-Cas12 and CRISPR-Cas13 have been introduced. CRISPR-Cas9 system has played a key role in the industrial cell factory's production and improved our understanding of genome function. Additionally, this system has been used as one of the major genome editing systems for the diagnosis and treatment of several infectious and non-infectious diseases. In this review, we discuss CRISPR biology, its versatility, and its application in biomedical engineering.
引用
收藏
页码:163 / 173
页数:11
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