Application of amperometric sol-gel biosensor to flow injection determination of glucose

被引:27
作者
Couto, CMCM
Araújo, AN
Montenegro, MCBSM
Rohwedder, J
Raimundo, I
Pasquini, C
机构
[1] CEQUP, Dept Quim Fis, Fac Farm, P-4050 Oporto, Portugal
[2] Univ Estadual Campinas, Inst Quim, Campinas, SP, Brazil
[3] Inst Super Ciencias Saude, P-4580 Gandra, Paredes, Portugal
关键词
flow injection analysis; glucose oxidase; sol-gel; glucose biosensor;
D O I
10.1016/S0039-9140(01)00587-2
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
A glucose biosensor with enzyme immobilised by sol-gel technology was constructed and evaluated. The glucose biosensor reported is based on encapsulated GOX within a sol-gel glass, prepared with 3-aminopropyltriethoxy silane, 2-(3,4-epoxycyclohexyl)-ethyltrimetoxy silane and HCl. A flow system incorporating the amperometric biosensor constructed was developed for the determination of glucose in the 1 x 10(-4)-5 x 10(-3) mol 1(-1) range with a precision of 1.5%. The results obtained for the analysis of electrolytic solution for iv administration and human serum samples showed good agreement between the proposed method and the reference procedure, with relative error < 5%. (C) 2002 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:997 / 1003
页数:7
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