Characterization of immobilized laccase from Lentinula edodes and its use in olive-mill wastewater treatment

被引：137

作者：

D'Annibale, A ^{[1
]}

Stazi, SR ^{[1
]}

Vinciguerra, V ^{[1
]}

Di Mattia, E ^{[1
]}

Sermanni, GG ^{[1
]}

机构：

[1] Univ Tuscia, Dept Agrobiol & Agrochem, I-01100 Viterbo, Italy

来源：

PROCESS BIOCHEMISTRY | 1999年 / 34卷 / 6-7期

关键词：

Lentinula edodes; laccase; immobilization; olive-mill wastewater; phenolic effluents; stabilization;

D O I：

10.1016/S0032-9592(98)00144-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

This study is mainly concerned with the characterization of an immobilized laccase from the white-rot fungus Lentinula edodes for use in wastewater treatment. The enzyme was immobilized on chitosan by adsorption and subsequent cross-linking with glutaraldehyde. The immobilized laccase displayed a lower specific activity and a lower substrate affinity than the free enzyme. Nevertheless, the immobilized catalyst exhibited an appreciable catalytic capability (520 units g(-1) support) along with remarkably improved stability properties to various parameters, such as temperature, pH and storage time. The treatment of olive mill wastewater with immobilized laccase led to a partial decolorization as well as to significant abatements in its content in polyphenols, ortho-diphenols combined with a decreased toxicity of the effluent. (C) 1999 Elsevier Science Ltd. All rights reserved.

引用

页码：697 / 706

页数：10

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