BIOANALYTICAL METHOD DEVELOPMENT AND VALIDATION OF PREGABALIN IN RAT PLASMA BY SOLID PHASE EXTRACTION WITH HPLC-MS/MS: APPLICATION TO A PHARMACOKINETIC STUDY

被引：6

作者：

Karavadi, Thejo Moorthy ^{[1
]}

Challa, B. R. ^{[2
]}

机构：

[1] Pacific Univ Higher Educ & Res, Udaipur, Rajestan, India

[2] Nirmala Coll Pharm, Dept Pharmaceut Chem, Kadapa 516002, Andhra Pradesh, India

来源：

JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES | 2014年 / 37卷 / 01期

关键词：

anticonvulsant; HPLC-MS; MS; pharmacokinetic study; pregabalin; rat plasma; solid-phase extraction; LC-MS-MS; FLUORESCENCE DETECTION; O-PHTALDIALDEHYDE; DERIVATIZATION; SEPARATION;

D O I：

10.1080/10826076.2012.738617

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The most suitable bioanalytical method based on solid phase extraction has been developed and validated for quantification of pregabalin in rat plasma. Pregabalin D4 was used as an internal standard for pregabalin. Thermo Hypurity C18 (4.6mmx150mm, 5.0 mu m) column provided chromatographic separation of analyte followed by detection with mass spectrometry. The method involved simple isocratic chromatographic separation and mass spectrometric detection in the positive ionization mode using an API-4000 system. The total run time was 3.5 minutes. The proposed method has been validated with the linear range of 0.50-20000.00ng/mL for Pregabalin. The intra-run and inter-run precision values were within 1.05 to 4.81% and 1.57 to 3.90%, respectively. The overall recovery for pregabalin and pregabalin D4 was 86.49% and 86.57%, respectively. This validated method was successfully applied into the pharmacokinetic study of rat plasma.

引用

页码：130 / 144

页数：15

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