Targeted Metabolomics of Dried Blood Spot Extracts

被引：86
作者：
Zukunft, Sven [1 ,2 ]
Sorgenfrei, Martina [1 ]
Prehn, Cornelia [1 ]
Moeller, Gabriele [1 ]
Adamski, Jerzy [1 ,2 ,3 ]
机构：
[1] German Res Ctr Environm Hlth, Helmholtz Zentrum Munchen, Genome Anal Ctr, Inst Expt Genet, D-85764 Neuherberg, Germany
[2] German Ctr Diabet Res, D-85764 Neuherberg, Germany
[3] Tech Univ Munich, D-80290 Munich, Germany
来源：
CHROMATOGRAPHIA | 2013年 / 76卷 / 19-20期
关键词：
Dried blood spots; Mass spectrometry; Targeted metabolomics; Lipids; Metabolite quantification; TANDEM MASS-SPECTROMETRY; AMINO-ACIDS; VALIDATION; ACYLCARNITINES; BIOMARKERS; STABILITY;
D O I：
10.1007/s10337-013-2429-3
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Dried blood spot (DBS) samples are already successfully used in newborn screening and pharmacological analyses. The application of DBS matrix to further metabolomic methods will considerably extend the analytical options for the diagnostics of metabolic diseases. We present an MS/MS based method for the simultaneous extraction and quantification of 188 metabolites from dried blood spots. We provide a sensitive and reproducible method that adapts the AbsoluteIDQ (TM) p180 kit of Biocrates to the DBS matrix for the quantification of metabolites of different substance classes including amino acids, biogenic amines, free carnitine, acylcarnitines, hexoses, glycerophospholipids, lysophosphatidylcholines, phosphatidylcholines, and sphingolipids.
引用
收藏
页码：1295 / 1305
页数：11
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