Effect of Interval between Human Chorionic Gonadotropin Priming and Ovum Pick-up on the Euploid Probabilities of Blastocyst

This retrospective study attempts to elucidate the relevance of the interval between human chorionic gonadotropin priming and oocyte pick-up (hCG-OPU) to the euploidy probability of biopsied blastocysts in preimplantation genetic tests for aneuploidy (PGT-A) cycles. A total of 1889 blastocysts from 511 patients undergoing PGT- A cycles were used. An analysis of generalized estimating equations (GEE) was used to identify whether the hCG-OPU interval is associated with euploidy probabilities of blastocysts. Accordingly, maternal age (OR: 0.925, 95% CI: 0.903-0.948,p< 0.001) and the hCG-OPU interval (OR: 1.138, 95% CI: 1.028-1.260,p= 0.013) were the two significant factors associated with the euploidy probabilities. The Cochran-Armitage trend test demonstrated that the blastocyst euploidy percentage increased progressively with the increasing hCG-OPU interval in normal responders (p= 0.006) and advanced maternal age (age >= 38 years;p= 0.020) groups. In normal responders, the euploidy rate was highest in the 38-39 h interval (43.1%, 47/109). In contrast, the euploidy rate was lowest in the 34-35 h interval (28.7%, 29/105). In conclusion, the present study demonstrated that at an hCG-OPU interval between 34-39 h, the longer the hCG-OPU interval, the higher the probability of euploidy for blastocysts.