Transforming growth factor (TGF)-β1-induced human endometrial stromal cell decidualization through extracellular signal-regulated kinase and Smad activation in vitro:: peroxisome proliferator-activated receptor gamma acts as a negative regulator of TGF-β1

被引：35

作者：

Chang, Hye Jin ^{[1
]}

Lee, Jae Hoon ^{[1
,2
]}

Hwang, Kyung Joo ^{[1
]}

Kim, Mi Ram ^{[1
]}

Chang, Ki Hong ^{[1
]}

Park, Dong Wook ^{[2
]}

Min, Churl K. ^{[2
]}

机构：

[1] Ajou Univ, Sch Med, Dept Obstet & Gynecol, Suwon 443749, South Korea

[2] Ajou Univ, Dept Mol Sci & Technol, Suwon 443749, South Korea

来源：

FERTILITY AND STERILITY | 2008年 / 90卷

关键词：

decidualization; TGF-beta; 1; PPAR gamma; ERK; PGE(2); Smad;

D O I：

10.1016/j.fertnstert.2007.09.010

中图分类号：

R71 [妇产科学];

学科分类号：

100211 ;

摘要：

Objective: To investigate the effect of transforming growth factor (TGF)-beta 1 on the extracellular signal-regulated kinase (ERK) and Smad pathway and the role of peroxisome proliferator-activated receptor (PPAR)-gamma in cultured human endometrial stromal cells. Design: Experimental study. Setting: Infertility center of a tertiary university hospital. Material(s): Human endometrial tissues obtained by hysterectomy from patients with conditions other than endometrial diseases. Intervention(S): Endometrial stromal cells were cultured under normal laboratory conditions. TGF-beta 1, rosiglitazone (PPAR gamma agonist), and PD98059 (ERK inhibitor) were added to endometrial stromal cell culture according to experimental purposes. Main Outcome Measure(s): Cell count, PRL expression, Smad and ERK phosphorylation, cyclooxygenase (COX)-2 expression, and prostaglandin E-2 (PGE(2)) release., Result(S): TGF-beta 1 inhibited cellular proliferation and induced the expressions of COX-2, PGE(2), and PRL of cultured human endometrial stromal cells. These effects may be mediated by Smad, and ERK phosphorylation. Treatment with rosiglitazone, a PPAR gamma agonist, reversed the TGF-beta 1 effect by antagonizing the activation of ERK and Smad that was induced by TGF-beta 1. Conclusion(s): PPAR gamma plays a negative role by directly acting. on Smad and ERK phosphorylation in human endometrial cell decidualization that is induced by TGF-beta 1 in vitro.

引用

页码：1357 / 1365

页数：9

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