The mechanism of ascorbic acid and hydroquinone cooxidation catalyzed by horseradish peroxidase

被引：0

作者：

Rogozhin, VV ^{[1
]}

Verkhoturov, VV ^{[1
]}

机构：

[1] Yakutsk State Agr Acad, Yakutsk 677002, Russia

来源：

BIOORGANICHESKAYA KHIMIYA | 1999年 / 25卷 / 05期

关键词：

ascorbic acid; horseradish peroxidase; hydroquinone;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

A steady-state kinetics of peroxidase cooxidation of ascorbic acid and hydroquinone catalyzed by horseradish peroxidase was studied. Ascorbic acid and hydroquinone were shown to be oxidized successively, and hydroquinone promoted the oxidation of ascorbic acid. Excess ascorbic acid inhibited peroxidase in the cooxidation of the substrates at pH 5-7. The values of catalytic constants (k(cat), K-m, and K-a) were determined. A possible activation mechanism of the peroxidation of ascorbic acid in the presence of hydroquinone was suggested, and its biological significance was considered.

引用

页码：377 / 382

页数：6

共 26 条

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