Removal of Anthracene from Recently Contaminated and Aged Soils

Remediation of anthracene from soil was faster in the topsoil than in the lower layers. It was not clear whether this was due to a higher anthracene bioavailability or an increased microbial degradation in the topsoil layer. Therefore, an arable soil was contaminated with 500 mg anthracene kg(-1) dry soil, and its removal was monitored with an exhaustive technique and with n-butanol to determine its bioavailability in three layers. Additionally, part of the contaminated soil was stored aerobically for 112 days (considered the aged soil), and the anthracene was monitored thereafter. After 112 days, 360 mg anthracene kg(-1) was removed from the soil, but its dissipation was not affected by the layer, depth, and fluctuations in water content or the interaction between. In the aged soil stored for 112 days, only 170 mg anthracene kg(-1) was removed. In the subsequent incubation of the aged soil, 273 mg anthracene kg(-1) was removed within 28 days, and after 112 days, no contaminant was detectable in the soil. An additional experiment confirmed the hypothesis that simply mixing the soil accelerated the removal of anthracene. Mixing the soil every 7 days removed all anthracene from the soil within 28 days, while in the undisturbed soil, 301 mg anthracene kg(-1) was still extractable. It can be concluded that mixing the soil accelerated the removal of anthracene, but fluctuations in water content did not. The anthracene extracted with n-butanol was not related to the amount removed and is thus not a good indicator of bioavailability of anthracene in soil.