Escheriosomes entrapped DNA vaccine co-expressing Cu-Zn superoxide dismutase and IL-18 confers protection against Brucella abortus

被引：19

作者：

Singha, H. ^{[2
]}

Mallick, A. I. ^{[1
]}

Jana, C. ^{[2
]}

Isore, D. P. ^{[2
]}

Goswami, T. K. ^{[2
]}

Srivastava, S. K. ^{[3
]}

Azevedo, V. A. ^{[4
]}

Chaudhuri, P. ^{[2
]}

Owais, M. ^{[1
]}

机构：

[1] Aligarh Muslim Univ, Interdisciplinary Biotechnol Unit, Aligarh 202002, Uttar Pradesh, India

[2] Indian Vet Res Inst, Div Biotechnol, Izatnagar 243122, Uttar Pradesh, India

[3] Indian Vet Res Inst, Div Bacteriol, Izatnagar 243122, Uttar Pradesh, India

[4] Inst Ciencial Biol, Depto Biol Geral, Minas Gerais, Brazil

来源：

MICROBES AND INFECTION | 2008年 / 10卷 / 10-11期

关键词：

Escheriosomes; Brucella abortus; Cu-Zn superoxide dismutase; IL-18;

D O I：

10.1016/j.micinf.2008.05.007

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

In the present study, we evaluated prophylactic prospective of liposome based DNA vaccine co-expressing Cu-Zn superoxide dismutase (SOD) along with interleukin-18 (IL-18) against experimental murine brucellosis. The immunization schedule involves liposome-mediated delivery of pVsod (encoding SOD of Brucella abortus) and pVIL18-sod (encoding IL-18 of mouse and SOD of B. abortus) DNA constructs. The data highlight potential of Escherichia coli lipid liposome (escheriosome) based DNA delivery vehicle to induce SOD specific humoral and cellular immune responses in the immunized mice. The co-expression of SOD along with IL-18 ensued in higher lymphoproliferative response and IFN-gamma production in comparison to the group of animals that were immunized with free form of SOD-DNA. Antibody response developed upon immunization with both DNA vaccines was of IgG2a type mainly. The results of the present study show that co-expression of IL-18 along with SOD polarized the antigen specific immune responses toward Th-1 direction, a desirable feature to control intracellular pathogens. (c) 2008 Elsevier Masson SAS. All rights reserved.

引用

页码：1089 / 1096

页数：8

共 37 条

[1] Liposome mediated antigen delivery leads to induction of CD8+ T lymphocyte and antibody responses against the V3 loop region of HIV gp120 [J].

Ahmad, N ;

Khan, MA ;

Owais, M .

CELLULAR IMMUNOLOGY, 2001, 210 (01) :49-55

[2]

AHN JH, 1997, J IMMUNOL, V159, P21

[3] Induction of immune response in BALB/c mice with a DNA vaccine encoding bacterioferritin or P39 of Brucella spp. [J].

Al-Mariri, A ;

Tibor, A ;

Mertens, P ;

De Bolle, X ;

Michel, P ;

Godfroid, JK ;

Walravens, K ;

Letesson, J .

INFECTION AND IMMUNITY, 2001, 69 (10) :6264-6270

[4]

ANGELA B, 2006, INFECT IMMUN, V74, P3967

[5]

[Anonymous], 1990, ANIMAL BRUCELLOSIS

[6] Immunoregulatory functions of interleukin 18 and its role in defense against bacterial pathogens [J].

Biet, F ;

Locht, C ;

Kremer, L .

JOURNAL OF MOLECULAR MEDICINE-JMM, 2002, 80 (03) :147-162

[7] ANTIGENS OF BRUCELLA-ABORTUS S19 IMMUNODOMINANT FOR BOVINE LYMPHOCYTES AS IDENTIFIED BY ONE-DIMENSIONAL AND 2-DIMENSIONAL CELLULAR IMMUNOBLOTTING [J].

BROOKSWORRELL, BM ;

SPLITTER, GA .

INFECTION AND IMMUNITY, 1992, 60 (06) :2459-2464

[8] Brucellosis: An overview [J].

Corbel, MJ .

EMERGING INFECTIOUS DISEASES, 1997, 3 (02) :213-221

[9]

ELZER PH, 1994, IMMUNOLOGY, V82, P651

[10] Immunity and protection against Brucella abortus [J].

Golding, B ;

Scott, DE ;

Scharf, O ;

Huang, LY ;

Zaitseva, M ;

Lapham, C ;

Eller, N ;

Golding, H .

MICROBES AND INFECTION, 2001, 3 (01) :43-48

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