Chances and pitfalls of chemical cross-linking with amine-reactive N-hydroxysuccinimide esters

被引：211
作者：
Kalkhof, Stefan [1 ]
Sinz, Andrea [1 ]
机构：
[1] Univ Halle Wittenberg, Inst Pharm, Dept Pharmaceut Chem & Bioanalyt, D-06120 Halle, Saale, Germany
来源：
ANALYTICAL AND BIOANALYTICAL CHEMISTRY | 2008年 / 392卷 / 1-2期
关键词：
chemical cross-linking; amine-reactive cross-linkers; mass spectrometry;
D O I：
10.1007/s00216-008-2231-5
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
In this report we summarize our experiences with the reaction products of N-hydroxysuccinimide (NHS) esters, which are widely used for chemical cross-linking of lysine residues in proteins. We describe the products, which should be scrutinized during data analysis using customized software when NHS esters are employed for chemical cross-linking. Reaction products of NHS esters were observed not only with lysines, but also with serines, tyrosines, and threonines. This report is intended to be a practical guide for those working in the field of chemical cross-linking and mass spectrometry.
引用
收藏
页码：305 / 312
页数：8
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