Partition and recovery of phytase from Absidia blakesleeana URM5604 using PEG-citrate aqueous two-phase systems

被引：19

作者：

Carvalho Neves, Maria Luiza ^{[1
,2
,3
]}

Porto, Tatiana Souza ^{[1
,4
]}

Souza-Motta, Cristina Maria ^{[5
]}

Spier, Michele Rigon ^{[6
]}

Soccol, Carlos Ricardo ^{[6
]}

Moreira, Keila Aparecida ^{[1
,4
]}

Figueiredo Porto, Ana Lucia ^{[1
,2
]}

机构：

[1] Univ Fed Rural Pernambuco, Dept Morphol & Anim Physiol, BR-52171900 Recife, PE, Brazil

[2] Renorbio UECE, BR-60740000 Fortaleza, CE, Brazil

[3] ICB UPE, Dept Pathol, BR-50100130 Recife, PE, Brazil

[4] UFRPE, Acad Unit Garanhuns, BR-55292270 Garanhuns, PE, Brazil

[5] Univ Fed Pernambuco, Dept Mycol, BR-50670901 Recife, PE, Brazil

[6] UFPR, Dept Biotechnol & Bioproc, BR-81531970 Curitiba, PR, Brazil

来源：

FLUID PHASE EQUILIBRIA | 2012年 / 318卷

关键词：

Aqueous two-phase system; Phytase; Partition; Recovery; PEG/citrate; SOLID-STATE FERMENTATION; PURIFICATION; EXTRACTION; PROTEASES; PATTERN; BROTH;

D O I：

10.1016/j.fluid.2012.01.004

中图分类号：

O414.1 [热力学];

学科分类号：

摘要：

The partition and recovery behavior of phytase, produced by solid-state cultivation utilizing citrus pulp as substrate, was determined in an aqueous two-phase system (ATPS), composed by polyethylene glycol (PEG-citrate). The 2(4) factorial design was conducted to evaluate the effect of the following independent variables: PEG molar mass, PEG and citrate concentrations and pH, while partition coefficient, recovery and purification factor were selected as the variables responses. The best recovery in phytase activity (115.24%) condition was obtained with 26% (w/w) PEG 8000 (g/mol), 12% (w/w) sodium citrate at pH 8.0, showing a purification factor of 1.35. However, the highest partition coefficient (14.42) was observed within a 26% (w/w) PEG 400 (g/mol) and a 20% (w/w) sodium citrate at pH 6.0. The independent variables which more influenced on the partition coefficient and recovery were citrate concentration and PEG mass molar, respectively. The results suggest that PEG-citrate ATPS is an interesting and efficient alternative to partition and recovery of phytase from fermentation broth produced by Absidia blakesleeana URM5604. (c) 2012 Elsevier B.V. All rights reserved.

引用

页码：34 / 39

页数：6

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