Hepatoprotective and Anti-Hepatitis C Viral Activity of Platycodon grandiflorum Extract on Carbon Tetrachloride-Induced Acute Hepatic Injury in Mice

被引：11

作者：

Kim, Tae-Won ^{[2
]}

Lim, Jong-Hwan ^{[1
]}

Song, In-Bae ^{[2
]}

Park, Sang-Jin ^{[1
]}

Yang, Jae-Won ^{[1
]}

Shin, Jung Cheul ^{[1
]}

Suh, Joo-Won ^{[3
]}

Son, Hwa-Young ^{[2
]}

Cho, Eun-Sang ^{[2
]}

Kim, Myoung-Seok ^{[4
]}

Lee, Sang-Wook ^{[1
]}

Kim, Jong-Woo ^{[1
]}

Yun, Hyo-In ^{[2
]}

机构：

[1] Adv Inst Convergence Technol, B&C Biopharm, Suwon 443759, Gyeonggi Do, South Korea

[2] Chungnam Natl Univ, Coll Vet Med, Taejon 305764, South Korea

[3] Myongji Univ, Div Biosci & Bioinformat, Yongin 449728, Gyeonggi Do, South Korea

[4] Jeollanamdo Dev Inst Tradit Korean Med, Jangheung 529851, Jeollanam Do, South Korea

来源：

JOURNAL OF NUTRITIONAL SCIENCE AND VITAMINOLOGY | 2012年 / 58卷 / 03期

关键词：

Platycodon grandilorum; carbon tetrachloride; hepatotoxicity; hepatitis C virus; VIRUS-INFECTION; IN-VITRO; INTERFERON; MECHANISMS; RIBAVIRIN; TOXICITY; RADICALS; SAPONINS; CELLS; RADIX;

D O I：

10.3177/jnsv.58.187

中图分类号：

R15 [营养卫生、食品卫生]; TS201 [基础科学];

学科分类号：

100403 ;

摘要：

The present study aims to evaluate the anti-HCV activity of hotwater extract from Platycodon grandiflorum (BC703) with HCV genotype 1b subgenomic replicon system and investigate its hepatoprotective activity on carbon tetrachloride (CCl4)-induced acute liver damage in mice. BC703 produced significant hepatoprotective effects against CCl4-induced acute hepatic injury by decreasing the activities of serum enzymes, nitric oxide and lipid peroxidation. Histopathological studies further substantiated the protective effect of BC703. Furthermore, BC703 inhibited the HCV RNA replication with an EC50 value and selective index (CC50/EC30) of 2.82 mu g/mL and above 35.46, respectively. However, digested BC703 using a simulated gastric juice showed poor protective effect against CCl4-induced hepatotoxicity in mice and decreased anti-HCV activity as compared to the intact BC703. Although further studies are necessary. BC703 may be a beneficial agent for the management of acute hepatic injury and chronic HCV infection.

引用

页码：187 / 194

页数：8

共 30 条

[1] Epidemiology of hepatitis C virus infection [J].

Alter, Miriam J. .

WORLD JOURNAL OF GASTROENTEROLOGY, 2007, 13 (17) :2436-2441

[2] ANTIOXIDANT DETERMINATIONS BY THE USE OF A STABLE FREE RADICAL [J].

BLOIS, MS .

NATURE, 1958, 181 (4617) :1199-1200

[3]

BRATTIN W J, 1985, Journal of Free Radicals in Biology and Medicine, V1, P27, DOI 10.1016/0748-5514(85)90026-1

[4] Industrial solvents and liver toxicity: Risk assessment, risk factors and mechanisms [J].

Brautbar, N ;

Williams, J .

INTERNATIONAL JOURNAL OF HYGIENE AND ENVIRONMENTAL HEALTH, 2002, 205 (06) :479-491

[5]

Che YH, 1992, J LANZHOU MED COLL, V18, P253

[6] 3 MODELS OF FREE RADICAL-INDUCED CELL INJURY [J].

COMPORTI, M .

CHEMICO-BIOLOGICAL INTERACTIONS, 1989, 72 (1-2) :1-56

[7] Dose- and time-dependent effects of luteolin on carbon tetrachloride-induced hepatotoxicity in mice [J].

Domitrovic, Robert ;

Jakovac, Hrvoje ;

Milin, Cedomila ;

Radosevic-Stasic, Biserka .

EXPERIMENTAL AND TOXICOLOGIC PATHOLOGY, 2009, 61 (06) :581-589

[8] Mechanism of action of interferon and ribavirin in treatment of hepatitis C [J].

Feld, JJ ;

Hoofnagle, JH .

NATURE, 2005, 436 (7053) :967-972

[9] Peginterferon alfa-2a plus ribavirin for chronic hepatitis C virus infection. [J].

Fried, MW ;

Shiffman, ML ;

Reddy, KR ;

Smith, C ;

Marinos, G ;

Goncales, FL ;

Haussinger, D ;

Diago, M ;

Carosi, G ;

Dhumeaux, D ;

Craxi, A ;

Lin, A ;

Hoffman, J ;

Yu, J .

NEW ENGLAND JOURNAL OF MEDICINE, 2002, 347 (13) :975-982

[10] Polysaccharide isolated from the radix of Platycodon grandiflorum selectively activates B cells and macrophages but not T cells [J].

Han, SB ;

Park, SH ;

Lee, KH ;

Lee, CW ;

Lee, SH ;

Kim, HC ;

Kim, YS ;

Lee, HS ;

Kim, HM .

INTERNATIONAL IMMUNOPHARMACOLOGY, 2001, 1 (11) :1969-1978

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