Antibacterial and antifungal activity of small protein of Indigofera oblongifolia leaves

被引:43
作者
Dahot, MU [1 ]
机构
[1] Univ Sindh, Inst Chem, Dept Biochem, Enzyme & Fermentat Biotechnol Res Lab, Jamshoro, Pakistan
关键词
antibacterial; antifungal; small protein; Indigofera oblongifolia; leaves; chromatography; electrophoresis;
D O I
10.1016/S0378-8741(98)00136-6
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Four fractions from the leaves of Indigofera oblongifolia were obtained on Sephadex G-25 column chromatography. A single bands of these fractions were detected on Poly-acrylamide SDS gel electrophoresis. An antibacterial action of small protein/peptide was tested against Escherichia coli, Klebsiella aerogenes, Kl. pneumoniae, Staphyllococcus aureus, and Bacillus subtilis. Peptide 3 showed strong inhibitory activity against B. subtilis and Aspergillus niger but clear zone of inhibition was also noted against A. fumigatus. Peptide 4 showed significant inhibitory zone against KI. pneumoniae, S. aureus, B. subtilis: A. fumigatus, A. niger and A. flavus. (C) 1999 Elsevier Science Ireland Ltd. All rights reserved.
引用
收藏
页码:277 / 282
页数:6
相关论文
共 12 条
[2]  
HAMES BD, 1986, GEL ELECTROPHORESIS, P27
[3]   ANTIFUNGAL ACTIVITY OF CHITIN-BINDING PR-4 TYPE PROTEINS FROM BARLEY-GRAIN AND STRESSED LEAF [J].
HEJGAARD, J ;
JACOBSEN, S ;
BJORN, SE ;
KRAGH, KM .
FEBS LETTERS, 1992, 307 (03) :389-392
[4]  
Jafari S.M.H., 1960, FLORA KARACHI, P191
[5]   PATHOGENESIS-RELATED PROTEINS OF PLANTS [J].
LINTHORST, HJM .
CRITICAL REVIEWS IN PLANT SCIENCES, 1991, 10 (02) :123-150
[6]  
LOURY OH, 1951, J BIOL CHEM, V193, P265
[7]   INCREASED DISEASE SUSCEPTIBILITY OF TRANSGENIC TOBACCO PLANTS WITH SUPPRESSED LEVELS OF PREFORMED PHENYLPROPANOID PRODUCTS [J].
MAHER, EA ;
BATE, NJ ;
NI, WT ;
ELKIND, Y ;
DIXON, RA ;
LAMB, CJ .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (16) :7802-7806
[8]  
Rasheed A., 1990, PAKISTAN J BIOCH, V23, P55
[9]   ISOLATION AND PARTIAL CHARACTERIZATION OF 2 ANTIFUNGAL PROTEINS FROM BARLEY [J].
ROBERTS, WK ;
SELITRENNIKOFF, CP .
BIOCHIMICA ET BIOPHYSICA ACTA, 1986, 880 (2-3) :161-170
[10]  
TERRAS FRG, 1992, J BIOL CHEM, V267, P15301