Protection of Taurine Against PFOS-Induced Neurotoxicity in PC12 Cells

被引:11
作者
Li, Chunna [1 ]
Liu, Xiaohui [1 ]
Liu, Qi [1 ]
Li, Shuangyue [1 ]
Li, Yachen [1 ]
Hu, Hong [2 ]
Shao, Jing [1 ]
机构
[1] Dalian Med Univ, Sch Publ Hlth, Dept Environm Hlth & Toxicol, Dalian 116044, Peoples R China
[2] Dalian Med Univ, Lab Med, Hosp 2, Dalian 116027, Peoples R China
来源
TAURINE 10 | 2017年 / 975卷
基金
中国国家自然科学基金;
关键词
Taurine; Perfluorooctane sulfonates (PFOS); PC12; cells; Neurotoxicity; Antioxidant; PERFLUOROALKYL SUBSTANCES; INDUCED APOPTOSIS; DEVELOPMENTAL TOXICITY; OXIDATIVE STRESS; NEURODEGENERATION; ASSOCIATION; ADOLESCENTS; SULFONATE; EXPOSURE; PATHWAY;
D O I
10.1007/978-94-024-1079-2_72
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
As a new member of persistent organic pollutants, the potent neurotoxicity of perfluorooctane sulfonates (PFOS) found in epidemiological studies and laboratory research has drawn increasing attention around the world. Previous studies showed that apoptosis driven by oxidative stress and autophagy were both observed in PFOS-induced toxicity. Taurine has been demonstrated to exert potent protections against oxidative stress as an efficient antioxidant. Whether taurine could protect against the PFOS neurotoxicity is not known. In the present study, PC12 cells were treated with several concentrations of PFOS (31.25, 250 mu M) for 24 h. 3-(4,5-Dimethyl-thiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) assay was applied to assess the cell viability. DCFH-DA detector was used to explore the production of ROS. Caspase 3 activity was used to reflect the possible apoptosis pathway. The lyso-tracker red dying was invited to evaluate the autophagy. Our data showed that taurine could significantly reverse the decreased viability and the increased ROS production in PC12 cells treated with PFOS. Moreover, the increased autophagy and apoptosis elicited by PFOS in PC12 cells could also be attenuated by taurine. Collectively, our results indicate that taurine may be an effective antioxidant in fighting against PFOS cytotoxicity and therefore could potentially serve as a preventative and therapeutic agent for environmental pollution-related toxicities.
引用
收藏
页码:907 / 916
页数:10
相关论文
共 23 条
  • [1] Roles of 3-nitrotyrosine- and 4-hydroxynonenal-modified brain proteins in the progression and pathogenesis of Alzheimer's disease
    Butterfield, D. Allan
    Reed, Tanea
    Sultana, Rukhsana
    [J]. FREE RADICAL RESEARCH, 2011, 45 (01) : 59 - 72
  • [2] Perfluoroalkyl substances in aquatic environment-comparison of fish and passive sampling approaches
    Cerveny, Daniel
    Grabic, Roman
    Fedorova, Ganna
    Grabicova, Katerina
    Turek, Jan
    Kodes, Vit
    Golovko, Oksana
    Zlabek, Vladimir
    Randak, Tomas
    [J]. ENVIRONMENTAL RESEARCH, 2016, 144 : 92 - 98
  • [3] Perfluoroalkyl substances and beta cell deficient diabetes
    Conway, Baqiyyah
    Innes, Karen E.
    Long, Dustin
    [J]. JOURNAL OF DIABETES AND ITS COMPLICATIONS, 2016, 30 (06) : 993 - 998
  • [4] Developmental toxicity of perfluorononanoic acid in mice
    Das, Kaberi P.
    Grey, Brian E.
    Rosen, Mitchell B.
    Wood, Carmen R.
    Tatum-Gibbs, Katoria R.
    Zehr, R. Daniel
    Strynar, Mark J.
    Lindstrom, Andrew B.
    Lau, Christopher
    [J]. REPRODUCTIVE TOXICOLOGY, 2015, 51 : 133 - 144
  • [5] Connexin 43 mediates PFOS-induced apoptosis in astrocytes
    Dong, Li
    Yang, Xiaoyan
    Gu, Wen
    Zhao, Kangfeng
    Ge, Huizheng
    Zhou, Jianjun
    Bai, Xuetao
    [J]. CHEMOSPHERE, 2015, 132 : 8 - 16
  • [6] Neurobehavioural effects of developmental toxicity
    Grandjean, Philippe
    Landrigan, Philip J.
    [J]. LANCET NEUROLOGY, 2014, 13 (03) : 330 - 338
  • [7] Mechanism underlying the antioxidant activity of taurine: prevention of mitochondrial oxidant production
    Jong, Chian Ju
    Azuma, Junichi
    Schaffer, Stephen
    [J]. AMINO ACIDS, 2012, 42 (06) : 2223 - 2232
  • [8] Taurine and Its Neuroprotective Role
    Kumari, Neeta
    Prentice, Howard
    Wu, Jang-Yen
    [J]. TAURINE 8, VOL 1: THE NERVOUS SYSTEM, IMMUNE SYSTEM, DIABETES AND THE CARDIOVASCULAR SYSTEM, 2013, 775 : 19 - 27
  • [9] Perfluorooctane sulfonate-induced apoptosis of cerebellar granule cells is mediated by ERK 1/2 pathway
    Lee, Youn Ju
    Lee, Hyun-Gyo
    Yang, Jae-Ho
    [J]. CHEMOSPHERE, 2013, 90 (05) : 1597 - 1602
  • [10] Antenatal taurine supplementation increases taurine content in intrauterine growth restricted fetal rat brain tissue
    Li, Fang
    Teng, Hui-Yun
    Liu, Jing
    Wang, Hua-Wei
    Zeng, Li
    Zhao, Li-Fang
    [J]. METABOLIC BRAIN DISEASE, 2014, 29 (03) : 867 - 871