Molecular characterization of noroviruses detected in diarrheic stools of Michigan and Wisconsin dairy calves: circulation of two distinct subgroups

被引：45

作者：

Wise, AG

Monroe, SS

Hanson, LE

Grooms, DL

Sockett, D

Maes, RK ^{[1
]}

机构：

[1] Michigan State Univ, Vet Med Ctr A45, Diagnost Ctr Populat & Anim Hlth, E Lansing, MI 48824 USA

[2] Michigan State Univ, Vet Med Ctr A45, Dept Microbiol & Mol Genet, E Lansing, MI 48824 USA

[3] Ctr Dis Control & Prevent, Viral Gastroenteritis Sect, Atlanta, GA 30333 USA

[4] Michigan State Univ, Dept Large Anim Clin Sci, E Lansing, MI 48824 USA

[5] Wisconsin Vet Diagnost Labs, Madison, WI 53705 USA

来源：

VIRUS RESEARCH | 2004年 / 100卷 / 02期

关键词：

norovirus; NLV; Jena; Newbury agent-2; bovine enteric calicivirus; RT-PCR;

D O I：

10.1016/j.virusres.2003.11.014

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Noroviruses have emerged as the leading worldwide cause of acute non-bacterial gastroenteritis in humans. The presence of noroviruses in diarrheic stool samples from calves on Michigan and Wisconsin dairy farms was investigated by RT-PCR. Norovirus-positive samples were found on all eight farms studied in Michigan and on 2 out of 14 farms in Wisconsin. Phylogenetic analyses of partial polymerase and capsid sequences, derived for a subset of these bovine noroviruses, showed that these strains formed a group which is genetically distinct from the human noroviruses, but more closely related to genogroup I than to genogroup II human noroviruses. Examination of 2 full and 10 additional partial capsid (ORF2) sequences of these bovine strains revealed the presence of two genetic subgroups or clusters of bovine noroviruses circulating on Michigan and Wisconsin farms. One subgroup is "Jena-like", the other "Newbury agent-2-like". (C) 2003 Elsevier B.V. All rights reserved.

引用

页码：165 / 177

页数：13

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