Crystal structure of the ectodomain of human transferrin receptor

被引：266
作者：
Lawrence, CM
Ray, S
Babyonyshev, M
Galluser, R
Borhani, DW
Harrison, SC
机构：
[1] Howard Hughes Med Inst, Boston, MA 02115 USA
[2] Childrens Hosp, Mol Med Lab, Boston, MA 02115 USA
[3] Harvard Univ, Sch Med, Dept Biol Chem & Mol Pharmacol, Boston, MA 02115 USA
来源：
SCIENCE | 1999年 / 286卷 / 5440期
关键词：
D O I：
10.1126/science.286.5440.779
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
The transferrin receptor (TfR) undergoes multiple rounds of clathrin-mediated endocytosis and reemergence at the cell surface, importing iron-loaded transferrin (Tf) and recycling apotransferrin after discharge of iron in the endosome. The crystal structure of the dimeric ectodomain of the human TfR, determined here to 3.2 angstroms resolution, reveals a three-domain subunit. One domain closely resembles carboxy- and aminopeptidases, and features of membrane glutamate carboxypeptidase can be deduced from the TfR structure. A model is proposed for Tf binding to the receptor.
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收藏
页码：779 / 782
页数：4
相关论文
共 40 条
[1]   INTERMOLECULAR DISULFIDE BONDS ARE NOT REQUIRED FOR THE EXPRESSION OF THE DIMERIC STATE AND FUNCTIONAL-ACTIVITY OF THE TRANSFERRIN RECEPTOR [J].
ALVAREZ, E ;
GIRONES, N ;
DAVIS, RJ .
EMBO JOURNAL, 1989, 8 (08) :2231-2240
[2]   APOLACTOFERRIN STRUCTURE DEMONSTRATES LIGAND-INDUCED CONFORMATIONAL CHANGE IN TRANSFERRINS [J].
ANDERSON, BF ;
BAKER, HM ;
NORRIS, GE ;
RUMBALL, SV ;
BAKER, EN .
NATURE, 1990, 344 (6268) :784-787
[3]   STRUCTURE OF HUMAN LACTOFERRIN - CRYSTALLOGRAPHIC STRUCTURE-ANALYSIS AND REFINEMENT AT 2.8-A RESOLUTION [J].
ANDERSON, BF ;
BAKER, HM ;
NORRIS, GE ;
RICE, DW ;
BAKER, EN .
JOURNAL OF MOLECULAR BIOLOGY, 1989, 209 (04) :711-734
[4]  
ANDERSSON S, 1989, J BIOL CHEM, V264, P8222
[5]  
[Anonymous], ACTA CRYSTALLOGR D
[6]   MOLECULAR-STRUCTURE OF SERUM TRANSFERRIN AT 3.3-A RESOLUTION [J].
BAILEY, S ;
EVANS, RW ;
GARRATT, RC ;
GORINSKY, B ;
HASNAIN, S ;
HORSBURGH, C ;
JHOTI, H ;
LINDLEY, PF ;
MYDIN, A ;
SARRA, R ;
WATSON, JL .
BIOCHEMISTRY, 1988, 27 (15) :5804-5812
[7]   A NEW ROLE FOR THE TRANSFERRIN RECEPTOR IN THE RELEASE OF IRON FROM TRANSFERRIN [J].
BALI, PK ;
ZAK, O ;
AISEN, P .
BIOCHEMISTRY, 1991, 30 (02) :324-328
[8]   CRYSTALLIZATION AND X-RAY-DIFFRACTION STUDIES OF A SOLUBLE FORM OF THE HUMAN TRANSFERRIN RECEPTOR [J].
BORHANI, DW ;
HARRISON, SC .
JOURNAL OF MOLECULAR BIOLOGY, 1991, 218 (04) :685-689
[9]  
BRUNGER AT, 1992, XPLOR VERSION 3 0 SY
[10]   Functional analysis of human/chicken transferrin receptor chimeras indicates that the carboxy-terminal region is important for ligand binding [J].
Buchegger, F ;
Trowbridge, IS ;
Liu, LFS ;
White, S ;
Collawn, JF .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1996, 235 (1-2) :9-17
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