Exercise alters the circadian rhythm of REV-ERB-α and downregulates autophagy-related genes in peripheral and central tissues

被引:9
作者
da Rocha, Alisson L. [1 ]
Pinto, Ana P. [2 ]
Bedo, Bruno L. S. [3 ]
Morais, Gustavo P. [1 ]
Oliveira, Luciana C. [1 ]
Carolino, Ruither O. G. [2 ]
Pauli, Jose R. [4 ]
Simabuco, Fernando M. [4 ]
de Moura, Leandro P. [4 ]
Ropelle, Eduardo R. [4 ]
Cintra, Dennys E. [4 ]
Rivas, Donato A. [5 ]
da Silva, Adelino S. R. [1 ,2 ]
机构
[1] Univ Sao Paulo, Postgrad Program Rehabil & Funct Performance, Ribeirao Preto Med Sch, USP, Ave Bandeirantes 3900, BR-14040907 Ribeirao Preto, SP, Brazil
[2] Univ Sao Paulo, Sch Phys Educ & Sport Ribeirao Preto, USP, Ribeirao Preto, SP, Brazil
[3] Univ Sao Paulo, Sch Phys Educ & Sport, Dept Sport, Sao Paulo, Brazil
[4] Univ Estadual Campinas, Sch Appl Sci, Lab Mol Biol Exercise LaBMEx, UNICAMP, Limeira, SP, Brazil
[5] Tufts Univ, Nutr Exercise Physiol & Sarcopenia Lab, Boston, MA 02111 USA
基金
巴西圣保罗研究基金会;
关键词
SKELETAL-MUSCLE; PROTEIN-DEGRADATION; INTERVAL; INTENSITY; ADAPTATIONS; METABOLISM; DISEASE; CLOCK; MICE;
D O I
10.1038/s41598-022-24277-4
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
The transcriptional repressor REV-ERB-alpha, encoded by Nuclear Receptor Subfamily 1 Group D Member 1 (Nr1d1), has been considered to play an essential role in the skeletal muscle oxidative capacity adaptation and muscle mass control. Also, this molecule regulates autophagy via the repression of autophagy-related genes both in skeletal muscle and brain regions. Classically, training programs based on endurance or strength characteristics enhance skeletal muscle mass content and/or oxidative capacity, leading to autophagy activation in several tissues. Thus, it seems that REV-ERB-alpha regulates similar responses induced by exercise. However, how this molecule responds to different exercise models/intensities in different tissues is still unclear. Therefore, the main aim was to characterize the responses of REV-ERB-alpha and autophagy-related genes to different exercise protocols (endurance/interval run/strength) in distinct tissues (gastrocnemius, soleus and hippocampus). Since REV-ERB-alpha presents a circadian rhythm, the analyses were performed in a time-course manner. The endurance and strength groups attenuated REV-ERB-alpha transcriptional response during the time course in gastrocnemius and soleus. Conversely, the interval group enhanced the Nr1d1 expression in the hippocampus. All protocols downregulated the REV-ERB-alpha protein levels in gastrocnemius following the exercise session with concomitant nuclear exclusion. The major autophagy-related genes presented downregulation after the exercise session in all analyzed tissues. Altogether, these results highlight that REV-ERB-alpha is extremely sensitive to physical exercise stimuli, including different models and intensities in skeletal muscle and the hippocampus.
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页数:13
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