On the Relationship of Protein and mRNA Dynamics in Vertebrate Embryonic Development

被引：152

作者：

Peshkin, Leonid ^{[1
]}

Wuehr, Martin ^{[1
,2
]}

Pearl, Esther ^{[3
]}

Haas, Wilhelm ^{[2
]}

Freeman, Robert M., Jr. ^{[1
]}

Gerhart, John C. ^{[4
]}

Klein, Allon M. ^{[1
]}

Horb, Marko ^{[3
]}

Gygi, Steven P. ^{[2
]}

Kirschner, Marc W. ^{[1
]}

机构：

[1] Harvard Univ, Sch Med, Dept Syst Biol, Boston, MA 02115 USA

[2] Harvard Univ, Sch Med, Dept Cell Biol, Boston, MA 02115 USA

[3] Marine Biol Lab, Natl Xenopus Resource, Woods Hole, MA 02543 USA

[4] Univ Calif Berkeley, Dept Mol & Cell Biol, Berkeley, CA 96704 USA

来源：

DEVELOPMENTAL CELL | 2015年 / 35卷 / 03期

关键词：

GENE-EXPRESSION; XENOPUS; QUANTIFICATION; PROTEOMICS; ACCURATE; GENOME; ACETYLATION;

D O I：

10.1016/j.devcel.2015.10.010

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

A biochemical explanation of development from the fertilized egg to the adult requires an understanding of the proteins and RNAs expressed over time during embryogenesis. We present a comprehensive characterization of protein and mRNA dynamics across early development in Xenopus. Surprisingly, we find that most protein levels change little and duplicated genes are expressed similarly. While the correlation between protein and mRNA levels is poor, a mass action kinetics model parameterized using protein synthesis and degradation rates regresses protein dynamics to RNA dynamics, corrected for initial protein concentration. This study provides detailed data for absolute levels of similar to 10,000 proteins and similar to 28,000 transcripts via a convenient web portal, a rich resource for developmental biologists. It underscores the lasting impact of maternal dowry, finds surprisingly few cases where degradation alone drives a change in protein level, and highlights the importance of transcription in shaping the dynamics of the embryonic proteome.

引用

页码：383 / 394

页数：12

共 35 条

[1]

Barton NicholasH., 2007, EVOLUTION

[2] Xenbase: gene expression and improved integration [J].

Bowes, Jeff B. ;

Snyder, Kevin A. ;

Segerdell, Erik ;

Jarabek, Chris J. ;

Azam, Kenan ;

Zorn, Aaron M. ;

Vize, Peter D. .

NUCLEIC ACIDS RESEARCH, 2010, 38 :D607-D612

[3]

BRACHET J, 1950, CHEM EMBRYOLOGY

[4] Modeling the dynamics of transcriptional gene regulatory networks for animal development [J].

de-Leon, Smadar Ben-Tabou ;

Davidson, Eric H. .

DEVELOPMENTAL BIOLOGY, 2009, 325 (02) :317-328

[5] Pervasive and Persistent Redundancy among Duplicated Genes in Yeast [J].

Dean, E. Jedediah ;

Davis, Jerel C. ;

Davis, Ronald W. ;

Petrov, Dmitri A. .

PLOS GENETICS, 2008, 4 (07)

[6]

Force A, 1999, GENETICS, V151, P1531

[7] FORMATION AND DETECTION OF RNA-DNA HYBRID MOLECULES IN CYTOLOGICAL PREPARATIONS [J].

GALL, JG ;

PARDUE, ML .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1969, 63 (02) :378-&

[8]

Gerhart J., 1997, CELLS EMBRYOS EVOLUT

[9] Exploiting polypharmacology for drug target deconvolution [J].

Gujral, Taranjit Singh ;

Peshkin, Leonid ;

Kirschner, Marc W. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2014, 111 (13) :5048-5053

[10]

GURDON JB, 1983, METHOD ENZYMOL, V101, P370

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