Application of Box-Behnken Experimental Design to Optimize the Extraction of Insecticidal Cry1Ac from Soil

被引：30
作者：
Li, Yan-Liang [1 ,2 ]
Fang, Zhi-Xiang [3 ]
You, Jing [1 ]
机构：
[1] Chinese Acad Sci, Guangzhou Inst Geochem, State Key Lab Organ Geochem, Guangzhou 510640, Guangdong, Peoples R China
[2] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
[3] Minist Environm Protect China, Nanjing Inst Environm Sci, Nanjing 210042, Jiangsu, Peoples R China
来源：
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2013年 / 61卷 / 07期
关键词：
response surface methodology; Box-Behnken design; Cry1Ac protein; transgenic Bt cotton; soil; THURINGIENSIS SUBSP KURSTAKI; BACILLUS-THURINGIENSIS; BT COTTON; PROTEIN; TOXIN; ADSORPTION; FIELD; CLAY; ENDOTOXIN; CROPS;
D O I：
10.1021/jf304970g
中图分类号：
S [农业科学];
学科分类号：
09 ;
摘要：
A validated method for analyzing Cry proteins is a premise to study the fate and ecological effects of contaminants associated with genetically engineered Bacillus thuringiensis crops. The current study has optimized the extraction method to analyze Cry1Ac protein in soil using a response surface methodology with a three-level-three-factor Box-I3ehnken experimental design (BBD). The optimum extraction conditions were at 21 degrees C and 630 rpm for 2 h. Regression analysis showed a good fit of the experimental data to the second-order polynomial model with a coefficient of determination of 0.96. The method was sensitive and precise with a method detection limit of 0.8 ng/g dry weight and relative standard deviations at 7.3%. Finally, the established method was applied for analyzing Cry1Ac protein residues in field-collected soil samples. Trace amounts of Cry1Ac protein were detected in the soils where transgenic crops have been planted for 8 and 12 years.
引用
收藏
页码：1464 / 1470
页数：7
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