Analysis of Defined Combinations of Monoclonal Antibodies in Anthrax Toxin Neutralization Assays and Their Synergistic Action

被引：14
|
作者：
Ngundi, Miriam M. [1 ]
Meade, Bruce D. [3 ]
Little, Stephen F. [2 ]
Quinn, Conrad P. [4 ]
Corbett, Cindi R. [5 ]
Brady, Rebecca A. [1 ]
Burns, Drusilla L. [1 ]
机构：
[1] US FDA, Ctr Biol Evaluat & Res, Bethesda, MD USA
[2] USA, Med Res Inst Infect Dis, Ft Detrick, MD 21702 USA
[3] Meade Biol, Hillsborough, NC USA
[4] Ctr Dis Control & Prevent, Natl Ctr Immunizat & Resp Dis, Atlanta, GA USA
[5] Publ Hlth Agcy Canada, Natl Microbiol Lab, Winnipeg, MB, Canada
来源：
CLINICAL AND VACCINE IMMUNOLOGY | 2012年 / 19卷 / 05期
关键词：
PROTECTIVE ANTIGEN; BACILLUS-ANTHRACIS; LETHAL TOXIN; IN-VITRO; INHALATIONAL ANTHRAX; RHESUS MACAQUES; FC-RECEPTORS; GUINEA-PIGS; VACCINE; IMMUNITY;
D O I：
10.1128/CVI.05714-11
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
Antibodies against the protective antigen (PA) component of anthrax toxin play an important role in protection against disease caused by Bacillus anthracis. In this study, we examined defined combinations of PA-specific monoclonal antibodies for their ability to neutralize anthrax toxin in cell culture assays. We observed additive, synergistic, and antagonistic effects of the antibodies depending on the specific antibody combination examined and the specific assay used. Synergistic toxin-neutralizing antibody interactions were examined in more detail. We found that one mechanism that can lead to antibody synergy is the bridging of PA monomers by one antibody, with resultant bivalent binding of the second antibody. These results may aid in optimal design of new vaccines and antibody therapies against anthrax.
引用
收藏
页码：731 / 739
页数：9
相关论文
共 50 条
  • [1] Combinations of Monoclonal Antibodies to Anthrax Toxin Manifest New Properties in Neutralization Assays
    Pohl, Mary Ann
    Rivera, Johanna
    Nakouzi, Antonio
    Chow, Siu-Kei
    Casadevall, Arturo
    INFECTION AND IMMUNITY, 2013, 81 (06) : 1880 - 1888
  • [2] Comparison of Three Anthrax Toxin Neutralization Assays
    Ngundi, Miriam M.
    Meade, Bruce D.
    Lin, Tsai-Lien
    Tang, Wei-Jen
    Burns, Drusilla L.
    CLINICAL AND VACCINE IMMUNOLOGY, 2010, 17 (06) : 895 - 903
  • [3] Efficient neutralization of anthrax toxin by chimpanzee monoclonal antibodies against protective antigen
    Chen, ZC
    Moayeri, M
    Zhou, YH
    Leppla, S
    Emerson, S
    Sebrell, A
    Yu, FJ
    Svitel, J
    Schuck, P
    St Claire, M
    Purcell, R
    JOURNAL OF INFECTIOUS DISEASES, 2006, 193 (05): : 625 - 633
  • [4] Monoclonal Antibodies for AAV Neutralization Assays
    Besir, Hueseyin
    Holzinger, Dana
    Hammer, Katharina
    Odenwald, Caroline
    Queitsch, Iris
    MOLECULAR THERAPY, 2020, 28 (04) : 391 - 391
  • [5] Analysis of the Fc Gamma Receptor-Dependent Component of Neutralization Measured by Anthrax Toxin Neutralization Assays
    Verma, Anita
    Ngundi, Miriam M.
    Meade, Bruce D.
    De Pascalis, Roberto
    Elkins, Karen L.
    Burns, Drusilla L.
    CLINICAL AND VACCINE IMMUNOLOGY, 2009, 16 (10) : 1405 - 1412
  • [6] Anthrax toxin specific monoclonal antibodies as potential passive vaccine
    不详
    IMMUNOBIOLOGY, 2005, 210 (6-8) : 481 - 482
  • [7] SYNERGISTIC NEUTRALIZATION OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 BY COMBINATIONS OF HUMAN MONOCLONAL-ANTIBODIES
    LAAL, S
    BURDA, S
    GORNY, MK
    KARWOWSKA, S
    BUCHBINDER, A
    ZOLLAPAZNER, S
    JOURNAL OF VIROLOGY, 1994, 68 (06) : 4001 - 4008
  • [8] Contribution of Fc fragment of monoclonal antibodies to tetanus toxin neutralization
    Ghotloo, Somayeh
    Amiri, Mohammad Mehdi
    Khoshnoodi, Jalal
    Abbasi, Ebrahim
    Jeddi-Tehrani, Mahmood
    Golsaz-Shirazi, Forough
    Shokri, Fazel
    NEUROTOXICITY RESEARCH, 2020, 37 (03) : 578 - 586
  • [9] Contribution of Fc fragment of monoclonal antibodies to tetanus toxin neutralization
    Somayeh Ghotloo
    Mohammad Mehdi Amiri
    Jalal Khoshnoodi
    Ebrahim Abbasi
    Mahmood Jeddi-Tehrani
    Forough Golsaz-Shirazi
    Fazel Shokri
    Neurotoxicity Research, 2020, 37 : 578 - 586
  • [10] NEUTRALIZATION OF TOXICITY OF TETANUS TOXIN INDUCED BY MONOCLONAL-ANTIBODIES
    YOSHIDA, T
    MIZUGUCHI, J
    NAGAOKA, F
    KONDO, S
    MATUHASI, T
    JAPANESE JOURNAL OF MEDICAL SCIENCE & BIOLOGY, 1984, 37 (04): : 197 - 197
12345