Separation of overlapped particles in digital holographic microscopy

被引：13
|
作者：
El Mallahi, Ahmed [1 ]
Dubois, Frank [1 ]
机构：
[1] Univ Libre Bruxelles, Micrograv Res Ctr, B-1050 Brussels, Belgium
来源：
OPTICS EXPRESS | 2013年 / 21卷 / 05期
关键词：
SEGMENTATION; TRANSFORM; NUCLEI;
D O I：
10.1364/OE.21.006466
中图分类号：
O43 [光学];
学科分类号：
070207 ; 0803 ;
摘要：
In this paper, we present a procedure to separate aggregates of overlapped particles in digital holograms, based on a focus plane analysis applied to each particle. The method can be applied either on phase or on amplitude objects, according that each object has a border in one focus plane. Numerical simulations are performed to quantify the robustness of the process by increasing the overlapping areas between the particles. The separation algorithm is successfully demonstrated experimentally on different types of aggregates. (c) 2013 Optical Society of America
引用
收藏
页码：6466 / 6479
页数：14
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