Separation of overlapped particles in digital holographic microscopy

被引：13

作者：

El Mallahi, Ahmed ^{[1
]}

Dubois, Frank ^{[1
]}

机构：

[1] Univ Libre Bruxelles, Micrograv Res Ctr, B-1050 Brussels, Belgium

来源：

OPTICS EXPRESS | 2013年 / 21卷 / 05期

关键词：

SEGMENTATION; TRANSFORM; NUCLEI;

D O I：

10.1364/OE.21.006466

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

In this paper, we present a procedure to separate aggregates of overlapped particles in digital holograms, based on a focus plane analysis applied to each particle. The method can be applied either on phase or on amplitude objects, according that each object has a border in one focus plane. Numerical simulations are performed to quantify the robustness of the process by increasing the overlapping areas between the particles. The separation algorithm is successfully demonstrated experimentally on different types of aggregates. (c) 2013 Optical Society of America

引用

页码：6466 / 6479

页数：14

共 34 条

[1] Three-dimensional velocity near-wall measurements by digital in-line holography: calibration and results [J].

Allano, Daniel ;

Malek, Mokrane ;

Walle, Francoise ;

Corbin, Frederic ;

Godard, Gilles ;

Coetmellec, Sebastien ;

Lecordier, Bertrand ;

Foucaut, Jean-Marc ;

Lebrun, Denis .

APPLIED OPTICS, 2013, 52 (01) :A9-A17

[2]

[Anonymous], 1999, Morphological Image Analysis: Principles and Applications

[3] GENERALIZING THE HOUGH TRANSFORM TO DETECT ARBITRARY SHAPES [J].

BALLARD, DH .

PATTERN RECOGNITION, 1981, 13 (02) :111-122

[4]

Beucher S., 1992, SCANNING MICROSCOPY, P28

[5]

Born M., 1980, ELECTROMAGNETIC THEO

[6] Hydrodynamic lift of vesicles under shear flow in microgravity [J].

Callens, N. ;

Minetti, C. ;

Coupier, G. ;

Mader, M. -A. ;

Dubois, F. ;

Misbah, C. ;

Podgorski, T. .

EPL, 2008, 83 (02)

[7] Segmentation of Clustered Nuclei With Shape Markers and Marking Function [J].

Cheng, Jierong ;

Rajapakse, Jagath C. .

IEEE TRANSACTIONS ON BIOMEDICAL ENGINEERING, 2009, 56 (03) :741-748

[8]

Diels J.C., 2014, Ultrashort Laser Pulse Phenomena

[9] Holographic deconvolution microscopy for high-resolution particle tracking [J].

Dixon, Lisa ;

Cheong, Fook Chiong ;

Grier, David G. .

OPTICS EXPRESS, 2011, 19 (17) :16410-16417

[10] Digital holographic microscopy for the three-dimensional dynamic analysis of in vitro cancer cell migration [J].

Dubois, Frank ;

Yourassowsky, Catherine ;

Monnom, Olivier ;

Legros, Jean-Claude ;

Debeir, Olivier ;

Van Ham, Philippe ;

Kiss, Robert ;

Decaestecker, Christine .

JOURNAL OF BIOMEDICAL OPTICS, 2006, 11 (05)

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