Modulation of a Photoswitchable Dual-Color Quantum Dot containing a Photochromic FRET Acceptor and an Internal Standard

被引：83

作者：

Diaz, Sebastian A. ^{[1
,2
]}

Giordano, Luciana ^{[1
]}

Jovin, Thomas M. ^{[1
]}

Jares-Erijman, Elizabeth A. ^{[2
]}

机构：

[1] Max Planck Inst Biophys Chem, Lab Cellular Dynam, D-37077 Gottingen, Germany

[2] Univ Buenos Aires, Fac Ciencias Exactas & Nat, Dept Quim Organ, CIHIDECAR,CONICET, RA-1428 Buenos Aires, DF, Argentina

来源：

NANO LETTERS | 2012年 / 12卷 / 07期

关键词：

Quantum dots; FRET; photochromism; internal standard; ratiometric imaging; RESONANCE ENERGY-TRANSFER; NANOPARTICLES; DIHETEROARYLETHENES; DIARYLETHENES; NANOCRYSTALS; MECHANISMS; MICROSCOPY; CELLS; SHELL;

D O I：

10.1021/nl301093s

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Photoswitchable semiconductor nanoparticles, quantum dots (QDs), couple the advantages of conventional QDs with the ability to reversibly modulate the QD emission, thereby improving signal detection by rejection of background signals. Using a simple coating methodology with polymers incorporating a diheteroarylethene photochromic FRET acceptor as well as a spectrally distinct organic fluorophore, photoswitchable QDs were prepared that are small, biocompatible, and feature ratiometric dual emission. With programmed irradiation, the fluorescence intensity ratio can be modified by up to similar to 100%.

引用

页码：3537 / 3544

页数：8

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