Ascorbate administration to normal and cholesterol-fed rats inhibits in vitro tears formation in serum and liver homogenates

被引:11
作者
Santillo, M
Mondola, P
Milone, A
Gioielli, A
Bifulco, M
机构
[1] CNR,IST CHIM MOLEC INTERESSE BIOL,ARCO,ITALY
[2] UNIV NAPLES,CEOS,CNR,DIPARTIMENTO BIOL PATOL CELL MOL,I-80138 NAPLES,ITALY
[3] UNIV REGGIO CALABRIA,DIPARTIMENTO MED SPERIMENTALE & CLIN,CATANZARO,ITALY
关键词
ascorbate; lipid peroxidation; hypercholesterolemia;
D O I
10.1016/0024-3205(96)00068-9
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
We have recently shown that ascorbate has a hypocholesterolemic and hypotriglyceridemic effect on rats fed a diet enriched with 1.5% cholesterol and 25% hydrogenated coconut oil (Nath diet) In this study we evaluated the effect of intraperitoneal ascorbate administration on susceptibility to lipoperoxidation either in rats fed standard or Nath diet. In normal rats ascorbate treatment decreased (p<0.05) the susceptibility to lipoperoxidation induced by incubation of serum for 24 hours with 2.2 mM Cu+-, without altering the normal serum fatty acid profile. In rats fed Nath diet we observed a reduced susceptibility of serum to Cu+--induced lipoperoxidation (36%), according with their low levels of serum unsaturated fatty acids (40% less than rats fed standard diet) In these animals ascorbate administration affects serum fatty acid profile leading to a decrese of S/U ratio from 1.6 to 1.2 without significantly modifying the susceptibility of serum to lipoperoxidation Moreover, the production of spontaneous lipid peroxides in liver homogenates, measured as TBARS levels, was strongly inhibited by ascorbate (p<0.01) in rats fed either standard or Nath diet These data indicate that ascorbate administration exerts an antioxidant effect and that in hypercholesterolemic rats, in addition to a lipid lowering effect, ascorbate exerts a protective role against the peroxidative damage of lipids
引用
收藏
页码:1101 / 1108
页数:8
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