Enhanced biodegradation of phenol in a novel cyclic activated sludge integrated with a rotating bed bioreactor in anoxic and peroxidase-mediated conditions

Cyclic activated sludge integrated with a rotating bed bioreactor (CASIR) was used for phenol biodegradation. The effects of phenol loading rate, mixed liquor suspended solids (MLSS) concentration, media filling ratio, hydraulic retention time (HRT) and salinity were investigated for phenol degradation and COD removal. In the second phase of the study, the microbial content of the bioreactor was induced by hydrogen peroxide injection for in situ generation of peroxidase. For investigating the above-mentioned parameters, the bioreactor was operated for 535 days and residual phenol, nitrate and COD were measured daily. The variation of the dehydrogenase activity and peroxidase activity of suspended biomass and attached film were also monitored during the bioreactor operation. Complete degradation of phenol at the loading rate of 667 g m(-3) d(-1) was achieved in anoxic conditions. Addition of media to the bioreactor to form active attached biofilm led to the increase in tolerance of the bioreactor on organic loading shocks. It was found that increasing the salinity of the wastewater did not affect the performance of the bioreactor. Investigating dehydrogenase activity proved that the attached biofilm was more involved in phenol degradation, compared with the suspended biomass. However, after switching to peroxidase-mediated conditions, the organic loading tolerance of the bioreactor considerably increased and complete degradation of phenol at the loading rate of 2000 g m(-3) d(-1) was reached. After adaptation of the microorganisms for hydrogen peroxide, the peroxidase activity of 290 U g(biomass)(-1) was observed in the bioreactor. Accordingly, the H2O2-induced microbial cells in cyclic activated sludge could be considered as a promising technique for enzymatic degradation of phenol and corresponding COD.