Expression of BMP-2 and Ets1 in BMP-2-stimulated mouse pre-osteoblast differentiation is regulated by microRNA-370

被引：43

作者：

Itoh, Tomohiro ^{[1
]}

Ando, Masashi ^{[1
]}

Tsukamasa, Yasuyuki ^{[1
]}

Akao, Yukihiro ^{[2
]}

机构：

[1] Kinki Univ, Fac Agr, Dept Fisheries, Lab Aquat Food Sci, Nara 6318505, Japan

[2] Gifu Univ, United Grad Sch Drug Discovery & Med Informat Sci, Gifu 5011193, Japan

来源：

FEBS LETTERS | 2012年 / 586卷 / 12期

关键词：

Non-coding RNA; MicroRNA-370; Osteoblast; Differentiation; BMP-2; Ets1; TRANSCRIPTION FACTORS; SIGNAL-TRANSDUCTION; RESPONSE ELEMENT; BINDING-PROTEIN; BONE; GENE; CELLS; PROMOTER; GROWTH; OSTEOPONTIN;

D O I：

10.1016/j.febslet.2012.04.014

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

MicroRNAs (miRs) regulate several biological functions such as cell growth, cell differentiation, and carcinogenesis, by binding to the 3'-untranslated regions (3'-UTR) of specific target genes, in order to repress translation or promote degradation of the transcribed mRNAs. In the present study, using microRNA array and in silico analyses, we found that miR-370 regulates the expression of bone morphogenetic protein-2 (BMP-2) and V-ets Erythroblastosis Virus E26 Oncogene Homolog 1 (Ets1) in BMP-2-stimulated murine pre-osteoblast MC3T3-E1 cell differentiation. The enforced expression of mature miR-370 in MC3T3-E1 cells or primary osteoblast cells remarkably attenuated BMP-2-induced pre-osteoblast differentiation. To ascertain the mechanisms underlying the regulation of osteoblast differentiation by miR-370, we hypothesized a BMP-2-Ets1-PTHrP feed-forward loop regulatory mechanism. (c) 2012 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：1693 / 1701

页数：9

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