One-pot green synthesis of carbon dots by using Saccharum officinarum juice for fluorescent imaging of bacteria (Escherichia coli) and yeast (Saccharomyces cerevisiae) cells

被引：320

作者：

Mehta, Vaibhavkumar N. ^{[1
]}

Jha, Sanjay ^{[2
]}

Kailasa, Suresh Kumar ^{[1
]}

机构：

[1] SV Natl Inst Technol, Dept Appl Chem, Surat 395007, India

[2] Navsari Agr Univ, Gujarat Agr Biotechnol Inst, Surat 395007, India

来源：

MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS | 2014年 / 38卷

关键词：

Saccharum officinarum juice; Fluorescence microscopy; DLS; TEM; Bacteria and yeast cells; NANOPARTICLES FACILE PREPARATION; QUANTUM DOTS; SELECTIVE DETECTION; NANODOTS; LOCALIZATION; NANOCRYSTALS; PROBES;

D O I：

10.1016/j.msec.2014.01.038

中图分类号：

TB3 [工程材料学]; R318.08 [生物材料学];

学科分类号：

0805 ; 080501 ; 080502 ;

摘要：

We are reporting highly economical plant-based hydrothermal method for one-pot green synthesis of water-dispersible fluorescent carbon dots (CDs) by using Saccharum officinarum juice as precursor. The synthesized CDs were characterized by UV-visible, fluorescence, Fourier transform infrared (FT-IR), dynamic light scattering (DLS), high-resolution transmission electron microscopic (HR-TEM), and laser scanning confocal microscopic techniques. The CDs are well dispersed in water with an average size of similar to 3 nm and showed bright blue fluorescence under UV-light (lambda(ex) = 365 nm). These CDs acted as excellent fluorescent probes in cellular imaging of bacteria (Escherichia coli) and yeast (Saccharomyces cerevisiae). (C) 2014 Elsevier B.V. All rights reserved.

引用

页码：20 / 27

页数：8

共 36 条

[1] Biosynthesis of biocompatible cadmium telluride quantum dots using yeast cells [J].

Bao, Haifeng ;

Hao, Na ;

Yang, Yunxia ;

Zhao, Dongyuan .

NANO RESEARCH, 2010, 3 (07) :481-489

[2] Tracking bio-molecules in live cells using quantum dots [J].

Chang, Yun-Pei ;

Pinaud, Fabien ;

Antelman, Joshua ;

Weiss, Shimon .

JOURNAL OF BIOPHOTONICS, 2008, 1 (04) :287-298

[3] A green and facile approach for the synthesis of water soluble fluorescent carbon dots from banana juice [J].

De, Bibekananda ;

Karak, Niranjan .

RSC ADVANCES, 2013, 3 (22) :8286-8290

[4] Easy Synthesis and Imaging Applications of Cross-Linked Green Fluorescent Hollow Carbon Nanoparticles [J].

Fang, Youxing ;

Guo, Shaojun ;

Li, Dan ;

Zhu, Chengzhou ;

Ren, Wen ;

Dong, Shaojun ;

Wang, Erkang .

ACS NANO, 2012, 6 (01) :400-409

[5] Deep tissue two-photon microscopy [J].

Helmchen, F ;

Denk, W .

NATURE METHODS, 2005, 2 (12) :932-940

[6] Synthesis of high-quality carbon nanodots from hydrophilic compounds: role of functional groups [J].

Hsu, Pin-Che ;

Chang, Huan-Tsung .

CHEMICAL COMMUNICATIONS, 2012, 48 (33) :3984-3986

[7] One-pot green synthesis of nitrogen-doped carbon nanoparticles as fluorescent probes for mercury ions [J].

Huang, Hong ;

Lv, Jing-Jing ;

Zhou, Dan-Ling ;

Bao, Ning ;

Xu, Yue ;

Wang, Ai-Jun ;

Feng, Jiu-Ju .

RSC ADVANCES, 2013, 3 (44) :21691-21696

[8]

Jia XF, 2012, NANOSCALE, V4, P5572, DOI [10.1039/c2nr31319g, 10.1039/c2ib31319g]

[9] Immunocytochemical localization of prohormone convertases PC1 and PC2 in the anuran pituitary gland: Subcellular localization in corticotrope and melanotrope cells [J].

Kurabuchi, S ;

Tanaka, S .

CELL AND TISSUE RESEARCH, 1997, 288 (03) :485-496

[10]

Lakowicz J.R., 2004, PRINCIPLES FLUORESCE, V2nd

← 1 2 3 4 →