Isolation of the lignan secoisolariciresinol diglucoside from flaxseed (Linum usitatissimum L.) by high-speed counter-current chromatography

被引：48
|
作者：
Degenhardt, A [1 ]
Habben, S [1 ]
Winterhalter, P [1 ]
机构：
[1] Tech Univ Carolo Wilhelmina Braunschweig, Inst Food Chem, DE-38106 Braunschweig, Germany
来源：
JOURNAL OF CHROMATOGRAPHY A | 2002年 / 943卷 / 02期
关键词：
Linum usitatissimum; counter-current chromatography; plant materials; secoisolariciresinol diglucoside; glucosides; lignans phytoestrogens; plant hormones;
D O I：
10.1016/S0021-9673(01)01467-4
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
High-speed counter-current chromatography was successfully used for the isolation and purification of secoisolariciresinol diglucoside, a bioactive lignan from flaxseed (Linum usitatissimum L.). The solvent system consisted of tert.-butylmethyl ether-n-butanol-acetonitrile-water (1:3:1:5). The purity and identity of the isolated compound was checked by high-performance liquid chromatography analysis in combination with mass spectrometry and NMR measurements. (C) 2002 Elsevier Science B.V. All rights reserved.
引用
收藏
页码：299 / 302
页数：4
相关论文
共 50 条
  • [21] Extraction, purification and animal model testing of an anti-atherosclerotic lignan secoisolarichiresinol diglucoside from flaxseed (Linum usitatissimum)
    Muir, AD
    Westcott, ND
    Prasad, K
    SECOND WORLD CONGRESS ON MEDICINAL AND AROMATIC PLANTS FOR HUMAN WELFARE WOCMAP-2: PHARMACOGNOSY, PHARMACOLOGY, PHYTOMEDICINES, TOXICOLOGY, 1999, (501): : 245 - 248
  • [22] Isolation and purification of clemastanin B and indigoticoside A from Radix Isatidis by high-speed counter-current chromatography
    Peng, JY
    Fan, GR
    Wu, YT
    JOURNAL OF CHROMATOGRAPHY A, 2005, 1091 (1-2) : 89 - 93
  • [23] Preparative isolation and purification of astaxanthin from the microalga Chlorococcum sp by high-speed counter-current chromatography
    Li, HB
    Chen, F
    JOURNAL OF CHROMATOGRAPHY A, 2001, 925 (1-2) : 133 - 137
  • [24] Preparative isolation and purification of lutein from the microalga Chlorella vulgaris by high-speed counter-current chromatography
    Li, HB
    Chen, F
    Zhang, TY
    Yang, FQ
    Xu, GQ
    JOURNAL OF CHROMATOGRAPHY A, 2001, 905 (1-2) : 151 - 155
  • [25] Isolation of two anthocyanin sambubiosides from bilberry (Vaccinium myrtillus) by high-speed counter-current chromatography
    Du, QZ
    Jerz, G
    Winterhalter, P
    JOURNAL OF CHROMATOGRAPHY A, 2004, 1045 (1-2) : 59 - 63
  • [26] Isolation and purification of honokiol and magnolol from cortex Magnoliae officinalis by high-speed counter-current chromatography
    Wang, X
    Wang, YQ
    Geng, YL
    Li, FW
    Zheng, CC
    JOURNAL OF CHROMATOGRAPHY A, 2004, 1036 (02) : 171 - 175
  • [27] Preparative isolation and purification of gastrodin from the Chinese medicinal plant Gastrodia elata by high-speed counter-current chromatography
    Li, HB
    Chen, F
    JOURNAL OF CHROMATOGRAPHY A, 2004, 1052 (1-2) : 229 - 232
  • [28] Preparative isolation and purification of capsaicinoids from Capsicum frutescens using high-speed counter-current chromatography
    Li, Fuwei
    Lin, Yunliang
    Wang, Xiao
    Geng, Yanling
    Wang, Daijie
    SEPARATION AND PURIFICATION TECHNOLOGY, 2009, 64 (03) : 304 - 308
  • [29] Preparative isolation and purification of coumarins from Peucedanum praeruptorum Dunn by high-speed counter-current chromatography
    Liu, RM
    Feng, L
    Sun, AL
    Kong, LY
    JOURNAL OF CHROMATOGRAPHY A, 2004, 1057 (1-2) : 89 - 94
  • [30] Effects of germination on tocopherol, secoisolarlciresinol diglucoside, cyanogenic glycosides and antioxidant activities in flaxseed (Linum usitatissimum L.)
    Li, Xiang
    Li, Jingyan
    Dong, Shan
    Li, Yufei
    Wei, Liping
    Zhao, Caicai
    Li, Junyi
    Liu, Xuebo
    Wang, Yutang
    INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY, 2019, 54 (07) : 2346 - 2354
12345