MiR-34a, miR-21 and miR-23a as potential biomarkers for coronary artery disease: a pilot microarray study and confirmation in a 32 patient cohort

被引：81

作者：

Han, Hui ^{[1
]}

Qu, Guangjin ^{[1
]}

Han, Chenghua ^{[2
]}

Wang, Yuhong ^{[1
]}

Sun, Tingting ^{[1
]}

Li, Fengqing ^{[1
]}

Wang, Junxiao ^{[1
]}

Luo, Shanshun ^{[1
]}

机构：

[1] Harbin Med Univ, Hosp 1, Dept Gerontol, Harbin 150001, Heilongjiang Pr, Peoples R China

[2] Harbin Med Univ, Dept Nat Prod Chem, Daqing, Peoples R China

来源：

EXPERIMENTAL AND MOLECULAR MEDICINE | 2015年 / 47卷

基金：

中国国家自然科学基金;

关键词：

MICRORNA EXPRESSION; CELL-PROLIFERATION; TUMOR-SUPPRESSOR; HUMAN HEART; CANCER; MICE; SERUM; ATHEROSCLEROSIS; OVEREXPRESSION; ANGIOGENESIS;

D O I：

10.1038/emm.2014.81

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The aim of this study was to investigate the expression of circulating microRNAs (miRNAs) in apolipoprotein E (apoE) knockout mice (apoE(-/-)) and to validate the role of these miRNAs in human coronary artery disease (CAD). Pooled plasma from 10 apoE(-/-) mice and 10 healthy C57BL/6 (B6) mice was used to perform the microarray analysis. The results showed that miR-34a, miR-21, miR-23a, miR-30a and miR-106b were differentially expressed in apoE(-/-) mice, and these expression changes were confirmed by real-time quantitative reverse-transcription PCR. Then, miR-34a, miR-21, miR-23a, miR-30a and miR-106b were detected in the plasma of 32 patients with CAD and of 20 healthy controls. Only miR-34a, miR-21 and miR-23a were significantly differentially expressed in the plasma of CAD patients (all P<0.01). In conclusion, miR-34a, miR-21 and miR-23a were elevated in CAD patients, which means that these miRNAs might serve as biomarkers of CAD development and progression.

引用

页码：e138 / e138

页数：7

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