Hyaluronic acid and its derivative as a multi-functional gene expression enhancer: Protection from non-specific interactions, adhesion to targeted cells, and transcriptional activation

被引：137

作者：

Ito, Tomoko

Iida-Tanaka, Naoko

Niidome, Takuro

Kawano, Takahito

Kubo, Koji

Yoshikawa, Kenichi

Sato, Toshinori

Yang, Zhihong

Koyama, Yoshiyuki ^{[1
]}

机构：

[1] Otsuma Womens Univ, Dept Text Sci, Chiyoda Ku, Tokyo, Japan

[2] Otsuma Womens Univ, Dept Food Sci, Tokyo, Japan

[3] Kyushu Univ, Fac Engn, Fukuoka 812, Japan

[4] Kyoto Univ, Grad Sch Sci, Dept Phys, Kyoto, Japan

[5] Keio Univ, Fac Sci & Technol, Kanagawa, Japan

来源：

JOURNAL OF CONTROLLED RELEASE | 2006年 / 112卷 / 03期

基金：

日本学术振兴会;

关键词：

transfection; hyaluronic acid; transcription; HMG;

D O I：

10.1016/j.jconrel.2006.03.013

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Hyaluronic acid (HA), a natural anionic mucopolysaccharide, can be deposited onto the cationic surface of DNA/polyethyleneimine (PEI) complexes to recharge the surface potential and reduce nonspecific interactions with proteins. HA can also be used as a ligand to target specific cell receptors. Furthermore, HA-coating enhanced the transcriptional activity of the plasmid/PEI complexes, probably by loosening the tight binding between DNA and PEI, which facilitated the approach of transcription factors. Amphoteric HA derivative having spermine side chains (Spn-HA) with a structure similar to HMG protein showed higher transcription-enhancing activity than HA. Plasmid/PEI/Spn-HA ternary complex exhibited 29-fold higher transgene expression efficiency than naked plasrnid/PEI complexes in CHO cells. (c) 2006 Elsevier B.V All rights reserved.

引用

页码：382 / 388

页数：7

共 15 条

[11] Nonviral vectors in the new millennium: Delivery barriers in gene transfer [J].