Hyaluronic acid and its derivative as a multi-functional gene expression enhancer: Protection from non-specific interactions, adhesion to targeted cells, and transcriptional activation

被引：137
作者：
Ito, Tomoko
Iida-Tanaka, Naoko
Niidome, Takuro
Kawano, Takahito
Kubo, Koji
Yoshikawa, Kenichi
Sato, Toshinori
Yang, Zhihong
Koyama, Yoshiyuki [1 ]
机构：
[1] Otsuma Womens Univ, Dept Text Sci, Chiyoda Ku, Tokyo, Japan
[2] Otsuma Womens Univ, Dept Food Sci, Tokyo, Japan
[3] Kyushu Univ, Fac Engn, Fukuoka 812, Japan
[4] Kyoto Univ, Grad Sch Sci, Dept Phys, Kyoto, Japan
[5] Keio Univ, Fac Sci & Technol, Kanagawa, Japan
来源：
JOURNAL OF CONTROLLED RELEASE | 2006年 / 112卷 / 03期
基金：
日本学术振兴会;
关键词：
transfection; hyaluronic acid; transcription; HMG;
D O I：
10.1016/j.jconrel.2006.03.013
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Hyaluronic acid (HA), a natural anionic mucopolysaccharide, can be deposited onto the cationic surface of DNA/polyethyleneimine (PEI) complexes to recharge the surface potential and reduce nonspecific interactions with proteins. HA can also be used as a ligand to target specific cell receptors. Furthermore, HA-coating enhanced the transcriptional activity of the plasmid/PEI complexes, probably by loosening the tight binding between DNA and PEI, which facilitated the approach of transcription factors. Amphoteric HA derivative having spermine side chains (Spn-HA) with a structure similar to HMG protein showed higher transcription-enhancing activity than HA. Plasmid/PEI/Spn-HA ternary complex exhibited 29-fold higher transgene expression efficiency than naked plasrnid/PEI complexes in CHO cells. (c) 2006 Elsevier B.V All rights reserved.
引用
收藏
页码：382 / 388
页数：7
相关论文
共 15 条
[1]   Association and dissociation characteristics of polymer/DNA complexes used for gene delivery [J].
Arigita, C ;
Zuidam, NJ ;
Crommelin, DJA ;
Hennink, WE .
PHARMACEUTICAL RESEARCH, 1999, 16 (10) :1534-1541
[2]  
Bustin M, 1996, PROG NUCLEIC ACID RE, V54, P35, DOI 10.1016/S0079-6603(08)60360-8
[3]   Decreased binding to proteins and cells of polymeric gene delivery vectors surface modified with a multivalent hydrophilic polymer and retargeting through attachment of transferrin [J].
Dash, PR ;
Read, ML ;
Fisher, KD ;
Howard, KA ;
Wolfert, M ;
Oupicky, D ;
Subr, V ;
Strohalm, J ;
Ulbrich, K ;
Seymour, LW .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2000, 275 (06) :3793-3802
[4]   A versatile system for receptor-mediated gene delivery permits increased entry of DNA into target cells, enhanced delivery to the nucleus and elevated rates of transgene expression [J].
Fisher, KD ;
Ulbrich, K ;
Subr, V ;
Ward, CM ;
Mautner, V ;
Blakey, D ;
Seymour, LW .
GENE THERAPY, 2000, 7 (15) :1337-1343
[5]   DNA COMPLEXES WITH POLYCATIONS FOR THE DELIVERY OF GENETIC MATERIAL INTO CELLS [J].
KABANOV, AV ;
KABANOV, VA .
BIOCONJUGATE CHEMISTRY, 1995, 6 (01) :7-20
[6]   Novel poly(ethylene glycol) derivatives with carboxylic acid pendant groups: synthesis and their protection and enhancing effect on non-viral gene transfection systems [J].
Koyama, Y ;
Ito, T ;
Matsumoto, H ;
Tanioka, A ;
Okuda, T ;
Yamaura, N ;
Aoyagi, H ;
Niidome, T .
JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION, 2003, 14 (06) :515-531
[7]  
Koyama Y, 2002, MACROMOL BIOSCI, V2, P251, DOI 10.1002/1616-5195(200208)2:6<251::AID-MABI251>3.0.CO
[8]  
2-6
[9]   Novel receptor-mediated gene delivery system comprising plasmid/protamine/sugar-containing polyanion ternary complex [J].
Maruyama, K ;
Iwasaki, F ;
Takizawa, T ;
Yanagie, H ;
Niidome, T ;
Yamada, E ;
Ito, T ;
Koyama, Y .
BIOMATERIALS, 2004, 25 (16) :3267-3273
[10]   HYALURONATE CAN FUNCTION AS A CELL-ADHESION MOLECULE AND CD44 PARTICIPATES IN HYALURONATE RECOGNITION [J].
MIYAKE, K ;
UNDERHILL, CB ;
LESLEY, J ;
KINCADE, PW .
JOURNAL OF EXPERIMENTAL MEDICINE, 1990, 172 (01) :69-75
12