Rapid Determination of Volatile Organic Compounds in Human Whole Blood Using Static Headspace Sampling with Gas Chromatography and Mass Spectrometry

被引：7

作者：

Lee, Ji-Young ^{[1
,2
]}

Kim, Seungki ^{[1
]}

Lee, Jong-Tae ^{[3
]}

Choi, Jong-Ho ^{[2
]}

Lee, Jeongae ^{[1
]}

Pyo, Heesoo ^{[1
]}

机构：

[1] Korea Inst Sci & Technol, Biomol Funct Res Ctr, Seoul 136791, South Korea

[2] Korea Univ, Dept Chem, Seoul 136701, South Korea

[3] Korea Univ, Dept Environm Hlth, Seoul 136701, South Korea

来源：

BULLETIN OF THE KOREAN CHEMICAL SOCIETY | 2012年 / 33卷 / 12期

关键词：

VOCs; Whole blood; Headspace; HS-GC-MS; SOLID-PHASE MICROEXTRACTION; HYDROCARBONS; OPTIMIZATION; CONTAMINANTS; PESTICIDES; TOXICITY; TOLUENE;

D O I：

10.5012/bkcs.2012.33.12.3963

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Headspace (HS) and headspace solid-phase microextraction (HS-SPME) were studied for extracting volatile organic compounds (VOCs) from whole blood, with chemical and instrumental variables being optimized for maximum sensitivity: incubation at 60 degrees C, equilibration for 30 mm, pH 11, and 2 mL injection volume. Both techniques provided accurate analyses, with detection limits of 0.05-0.1 ng mL(-1) and 0.05-0.5 ng mL(-1). HS showed better sensitivity, reproducibility, and analysis times than HS-SPME. Overall levels of chloroform in whole blood were found to be 0.05-5.84 ng mL(-1); detected levels of benzene were 0.05-2.20 ng mL(-1).

引用

页码：3963 / 3970

页数：8

共 30 条

[1] Simultaneous determination of 60 pesticides in water using solid-phase microextraction and gas chromatography mass spectrometry [J].

BoydBoland, AA ;

Magdic, S ;

Pawliszyn, JB .

ANALYST, 1996, 121 (07) :929-937

[2] OPTIMIZATION OF SOLID-PHASE MICROEXTRACTION CONDITIONS FOR DETERMINATION OF PHENOLS [J].

BUCHHOLZ, KD ;

PAWLISZYN, J .

ANALYTICAL CHEMISTRY, 1994, 66 (01) :160-167

[3] DETERMINATION OF METHYLMERCURY IN FISH AND RIVER WATER SAMPLES USING IN-SITU SODIUM TETRAETHYLBORATE DERIVATIZATION FOLLOWING BY SOLID-PHASE MICROEXTRACTION AND GAS-CHROMATOGRAPHY MASS-SPECTROMETRY [J].

CAI, Y ;

BAYONA, JM .

JOURNAL OF CHROMATOGRAPHY A, 1995, 696 (01) :113-122

[4] Sensitive headspace gas chromatography-mass spectrometry determination of trihalomethanes in urine [J].

Caro, J. ;

Serrano, A. ;

Gallego, M. .

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2007, 848 (02) :277-282

[5]

CHAPMAN DE, 1990, DRUG METAB DISPOS, V18, P929

[6] DOG LIVER MICROSOMAL P450 ENZYME-MEDIATED TOLUENE BIOTRANSFORMATION [J].

HANIOKA, H ;

HAMAMURA, M ;

KAKINO, K ;

OGATA, H ;

JINNO, H ;

TAKAHASHI, A ;

NISHIMURA, T ;

ANDO, M .

XENOBIOTICA, 1995, 25 (11) :1207-1217

[7] Benzene-induced cancers: Abridged history and occupational health impact [J].

Huff, James .

INTERNATIONAL JOURNAL OF OCCUPATIONAL AND ENVIRONMENTAL HEALTH, 2007, 13 (02) :213-221

[8]

Kolb B., 1997, STATIC HEADSPACE GAS

[9] NEW IR FIBEROPTIC CHEMICAL SENSOR FOR IN-SITU MEASUREMENTS OF CHLORINATED HYDROCARBONS IN WATER [J].

KRSKA, R ;

TAGA, K ;

KELLNER, R .

APPLIED SPECTROSCOPY, 1993, 47 (09) :1484-1487

[10] Optimization of solid-phase microextraction for the speciation of butyl- and phenyltins using experimental designs [J].

Lespes, G ;

Desauziers, V ;

Montigny, C ;

Potin-Gautier, M .

JOURNAL OF CHROMATOGRAPHY A, 1998, 826 (01) :67-76

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