A novel method for banking dental pulp stem cells

被引：44

作者：

Gioventu, Silvia ^{[2
]}

Andriolo, Gabriella ^{[1
]}

Bonino, Ferruccio ^{[3
]}

Frasca, Stefania ^{[1
]}

Lazzari, Lorenza ^{[1
]}

Montelatici, Elisa ^{[1
]}

Santoro, Franco ^{[2
]}

Rebulla, Paolo ^{[1
]}

机构：

[1] IRCCS Fdn Ca Granda Osped Maggiore Policlin, Ctr Transfus Med Cellular Therapy & Cryobiol, I-20122 Milan, Italy

[2] Univ Milan, IRCCS Fdn Ca Granda Osped Maggiore Policlin, Fac Dent, Dept Surg Sci Reconstruct & Diagnost, I-20122 Milan, Italy

[3] IRCCS Fdn Ca Granda Osped Maggiore Policlin, Sci Direct, I-20122 Milan, Italy

来源：

TRANSFUSION AND APHERESIS SCIENCE | 2012年 / 47卷 / 02期

关键词：

Dental pulp stem cells; Human deciduous teeth; Nd:YAG laser; EXFOLIATED DECIDUOUS TEETH; REGENERATIVE MEDICINE; STEM/PROGENITOR CELLS; TOOTH REGENERATION; SPINAL-CORD; IN-VITRO; TISSUE; CRYOPRESERVATION; DIFFERENTIATION; BONE;

D O I：

10.1016/j.transci.2012.06.005

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Dental pulp stem cells (DPSC), a cell type of mesenchymal origin showing high proliferation and plasticity, are an emerging source of adult stem cells offering interesting features in view of potential applications in regenerative medicine. These features prompted us to develop a new method to cryopreserve DPSC inside a whole tooth, thus avoiding the need to purify the cells before cryopreservation and reducing the initial costs and workload of tooth banking. In this study we cryopreserved 4 human deciduous whole teeth after digging microchannels into the tooth with an Nd:YAG laser beam (laser piercing) to allow the cryopreservative to reach the dental pulp and preserve the cells at 80 C. Then, we isolated, expanded and characterized in vitro the stem cells after tooth thawing and mechanical fracture. In parallel, we characterized cells extracted from 2 teeth cryopreserved without laser piercing and from 4 non cryopreserved, non laser pierced, freshly fractured teeth. Our data demonstrate that DPSC isolated from laser pierced cryopreserved teeth show mesenchymal stem cells morphology, immunophenotype, viability and proliferation rate similar to those of cells isolated from fresh, non cryopreserved teeth, whereas significant loss of cell viability and proliferation rate was shown by cells isolated from teeth cryopreserved without laser piercing. These data support the use of this method for prospective whole tooth banking. (C) 2012 Elsevier Ltd. All rights reserved.

引用

页码：199 / 206

页数：8

共 52 条

[1] Adult human dental pulp stem cells differentiate toward functionally active neurons under appropriate environmental cues [J].