Lectin-induced alterations of the interaction of insulin and insulin-like growth factor 1 receptors with their ligands

被引：4

作者：

Masnikosa, Romana ^{[1
]}

Nikolic, Anna J. ^{[1
]}

Nedic, Olgica ^{[1
]}

机构：

[1] Univ Belgrade, INEP Inst Appl Nucl Energy, Belgrade 11080, Serbia

来源：

JOURNAL OF THE SERBIAN CHEMICAL SOCIETY | 2008年 / 73卷 / 8-9期

关键词：

IGF1R; IGF2R; IR; WGA; Con A; PHA;

D O I：

10.2298/JSC0809793M

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

In order to study whether the carbohydrate moieties of the human placental IGF-I receptor (IGF1R), IGF-II receptor (IGF2R) and insulin receptors (IRs) play a role in ligand binding, solubilised cell membrane preparations were incubated with (125)l-labelled IGF-I, IGF-II and insulin in the presence of lectins with different sugar specificities. Three incubation procedures were tested: ligand-first, co-incubation and lectin-first incubation. Wheat genii agglutinin (WGA), concanavalin A (Con A) and phytohaemagglutinin (PHA) altered the binding of IGF-I and insulin to their high-affinity receptors in a lectin specific and dose-dependent manner. whereas these jectins did not affect the interaction of IGF-II with its receptor(s). Moreover, the same lectins either inhibited or enhanced IGF-I and insulin binding, depending on the incubation scheme. These results also suggest that IR-A and IR-B from human placenta might be differently glycosylated.

引用

页码：793 / 804

页数：12

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