Paradoxical Decrease of an Adipose-Specific Protein, Adiponectin, in Obesity (Reprinted from BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, vol 257, pg 79-83, 1999)

被引：136

作者：

Arita, Yukio ^{[1
]}

Kihara, Shinji ^{[1
]}

Ouchi, Noriyuki ^{[1
]}

Takahashi, Masahiko ^{[1
]}

Maeda, Kazuhisa ^{[1
]}

Miyagawa, Jun-ichiro ^{[1
]}

Hotta, Kikuko ^{[1
]}

Shimomura, Iichiro ^{[1
]}

Nakamura, Tadashi ^{[1
]}

Miyaoka, Koji ^{[1
]}

Kuriyama, Hiroshi ^{[1
]}

Nishida, Makoto ^{[1
]}

Yamashita, Shizuya ^{[1
]}

Okubo, Kosaku ^{[2
]}

Matsubara, Kenji ^{[2
]}

Muraguchi, Masahiro ^{[3
]}

Ohmoto, Yasuichi ^{[3
]}

Funahashi, Tohru ^{[1
]}

Matsuzawa, Yuji ^{[1
]}

机构：

[1] Osaka Univ, Grad Sch Med, Dept Internal Med & Mol Sci, Suita, Osaka 5650871, Japan

[2] Osaka Univ, Inst Mol & Cellular Biol, Suita, Osaka 5650871, Japan

[3] Otsuka Pharmaceut Co Ltd, Cellular Technol Inst, Tokushima 77101, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2012年 / 425卷 / 03期

关键词：

EXPRESSION; GENE; CLONING;

D O I：

10.1016/j.bbrc.2012.08.024

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We isolated the human adipose-specific and most abundant gene transcript, apM1 (Maeda, K, et al., Biochem. Biophys. Res. Commun. 221, 286-289, 1996). The apM1 gene product was a kind of soluble matrix protein, which we named adiponectin. To quantitate the plasma adiponectin concentration, we have produced monoclonal and polyclonal antibodies for human adiponectin and developed an enzyme-linked immunosorbent assay (ELISA) system. Adiponectin was abundantly present in the plasma of healthy volunteers in the range from 1.9 to 17.0 mg/ml. Plasma concentrations of adiponectin in obese subjects were significantly lower than those in non-obese subjects, although adiponectin is secreted only from adipose tissue. The ELISA system developed in this study will be useful for elucidating the physiological and pathophysiological role of adiponectin in humans. (C) 1999 Academic Press

引用

页码：560 / 564

页数：5

共 10 条

[1] Paradoxical decrease of an adipose-specific protein, adiponectin, in obesity
Arita, Y
Kihara, S
Ouchi, N
Takahashi, M
Maeda, K
Miyagawa, J
Hotta, K
Shimomura, I
Nakamura, T
Miyaoka, K
Kuriyama, H
Nishida, M
Yamashita, S
Okubo, K
Matsubara, K
Muraguchi, M
Ohmoto, Y
Funahashi, T
Matsuzawa, Y
[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1999, 257 (01) : 79 - 83
[2] Development of radioimmunoassay for human leptin
Hosoda, K
Masuzaki, H
Ogawa, Y
Miyawaki, T
Hiraoka, J
Hanaoka, I
Yasuno, A
Nomura, T
Fujisawa, Y
Yoshimasa, Y
Nishi, S
Yamori, Y
Nakao, K
[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1996, 221 (02) : 234 - 239
[3] ADIPOSE EXPRESSION OF TUMOR-NECROSIS-FACTOR-ALPHA - DIRECT ROLE IN OBESITY-LINKED INSULIN RESISTANCE
HOTAMISLIGIL, GS
SHARGILL, NS
SPIEGELMAN, BM
[J]. SCIENCE, 1993, 259 (5091) : 87 - 91
[4] AdipoQ is a novel adipose-specific gene dysregulated in obesity
Hu, E
Liang, P
Spiegelman, BM
[J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 1996, 271 (18) : 10697 - 10703
[5] Analysis of an expression profile of genes in the human adipose tissue
Maeda, K
Okubo, K
Shimomura, I
Mizuno, K
Matsuzawa, Y
Matsubara, K
[J]. GENE, 1997, 190 (02) : 227 - 235
[6] CDNA cloning and expression of a novel adipose specific collagen-like factor, apM1 (Adipose most abundant gene transcript 1)
Maeda, K
Okubo, K
Shimomura, I
Funahashi, T
Matsuzawa, Y
Matsubara, K
[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1996, 221 (02) : 286 - 289
[7] Nakano Y, 1996, J BIOCHEM-TOKYO, V120, P803
[8] A NOVEL SERUM-PROTEIN SIMILAR TO C1Q, PRODUCED EXCLUSIVELY IN ADIPOCYTES
SCHERER, PE
WILLIAMS, S
FOGLIANO, M
BALDINI, G
LODISH, HF
[J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (45) : 26746 - 26749
[9] Enhanced expression of PAI-1 in visceral fat: Possible contributor to vascular disease in obesity
Shimomura, I
Funahashi, T
Takahashi, M
Maeda, K
Kotani, K
Nakamura, T
Yamashita, S
Miura, M
Fukuda, Y
Takemura, K
Tokunaga, K
Matsuzawa, Y
[J]. NATURE MEDICINE, 1996, 2 (07) : 800 - 803
[10] POSITIONAL CLONING OF THE MOUSE OBESE GENE AND ITS HUMAN HOMOLOG
ZHANG, YY
PROENCA, R
MAFFEI, M
BARONE, M
LEOPOLD, L
FRIEDMAN, JM
[J]. NATURE, 1994, 372 (6505) : 425 - 432

← 1 →