On-chip impedimetric detection of bacteriophages in dairy samples

被引:24
作者
Garcia-Aljaro, Cristina [1 ]
Munoz-Berbel, Xavier [1 ]
Munoz, Francisco J. [1 ]
机构
[1] Esfera UAB, CSIC, IMB CNM, Bellaterra 08193, Spain
关键词
Impedance spectroscopy; Whole-cell biosensor; Bacteriophage detection; Escherichia coli; BIOFILM FORMATION; DRINKING-WATER; PCR; IMPEDANCE; BACTERIA;
D O I
10.1016/j.bios.2008.08.047
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
Bacteriophage infection of starter cultures constitutes a major problem in the dairy fermentation industry, which may bring about important economic losses. In this study, a rapid detection method of bacteriophages was developed based on analysis of impedance changes occurred upon infection of a host-biofilm established onto metal microelectrodes. Bacteriophage PhiX174 and Escherichia coli WG5 were chosen as models for bacteriophage and host strain, respectively, because of their easiness of manipulation. Impedimetric changes occurring at the microelectrocle surface. caused by bacteriophage infection and subsequent lysis of the host strain, were monitored over a 6-h period after the initial inoculation of phages by non-faradic impedance spectroscopy (IS) in PBS and milk samples. Analysis of data was performed by two different approaches: (1) the equivalent circuit modelling theory, where a decrease in the magnitude of both the double layer and the biofilm capacitances due to the bacteriophage infection process was recorded, and (2) analysis of the impedance value, specially the impedance imaginary component (Z(i)) at selected frequencies. Zi is related to the capacitance of the circuit and also showed a decrease with respect to the control sample (without bacteriophages). The simplicity of the assay and the possibility of miniaturization of the system as well as its wide application, being able of detecting any bacteriophage as long as a suitable bacterial host is available, increase the number of applications to which this system could be used for. (C) 2008 Elsevier B.V. All rights reserved.
引用
收藏
页码:1712 / 1716
页数:5
相关论文
共 18 条
[1]  
ADAMS MARK H., 1959
[2]  
[Anonymous], 2000, ISO 10705-2
[3]   Characterization of type 2 quorum sensing in Klebsiella pneumoniae and relationship with biofilm formation [J].
Balestrino, D ;
Haagensen, JAJ ;
Rich, C ;
Forestier, C .
JOURNAL OF BACTERIOLOGY, 2005, 187 (08) :2870-2880
[4]   Bacteriophage-resistance systems in dairy starter strains: molecular analysis to application [J].
Coffey, A ;
Ross, RP .
ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY, 2002, 82 (1-4) :303-321
[5]   Multiplex PCR for the detection and identification of dairy bacteriophages in milk [J].
del Rio, B. ;
Binetti, A. G. ;
Martin, M. C. ;
Fernandez, M. ;
Magadan, A. H. ;
Alvarez, M. A. .
FOOD MICROBIOLOGY, 2007, 24 (01) :75-81
[6]   Detection of bacteriophage infection and prophage induction in bacterial cultures by means of electric DNA chips [J].
Gabig-Ciminska, M ;
Los, M ;
Holmgren, A ;
Albers, J ;
Czyz, A ;
Hintsche, R ;
Wegrzyn, G ;
Enfors, SO .
ANALYTICAL BIOCHEMISTRY, 2004, 324 (01) :84-91
[7]   PRACTICAL DIRECT PLAQUE-ASSAY FOR COLIPHAGES IN 100-ML SAMPLES OF DRINKING-WATER [J].
GRABOW, WOK ;
COUBROUGH, P .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1986, 52 (03) :430-433
[8]   ELECTRODE POTENTIAL GRADIENTS AND CELL DESIGN IN CONTROLLED POTENTIAL ELECTROLYSIS EXPERIMENTS [J].
HARRAR, JE ;
SHAIN, I .
ANALYTICAL CHEMISTRY, 1966, 38 (09) :1148-&
[9]   Biofilm susceptibility to bacteriophage attack: the role of phage-borne polysaccharide depolymerase [J].
Hughes, KA ;
Sutherland, IW ;
Jones, MV .
MICROBIOLOGY-UK, 1998, 144 :3039-3047
[10]  
LACZKA O, 2006, BIOSENS BIOELECTRON, V22, P1205