Identification and first insights into the structure and biosynthesis of chitin from the freshwater sponge Spongilla lacustris

被引：78

作者：

Ehrlich, Hermann ^{[1
,3
]}

Kaluzhnaya, Oksana V. ^{[2
,3
]}

Brunner, Eike ^{[3
]}

Tsurkan, Mikhail V. ^{[4
,5
]}

Ereskovsky, Alexander ^{[6
]}

Ilan, Micha ^{[7
]}

Tabachnick, Konstantin R. ^{[8
]}

Bazhenov, Vasilii V. ^{[1
]}

Paasch, Silvia ^{[3
]}

Kammer, Martin ^{[3
]}

Born, Rene ^{[9
]}

Stelling, Allison ^{[10
]}

Galli, Roberta ^{[10
]}

Belikov, Sergei ^{[2
]}

Petrova, Olga V. ^{[11
]}

Sivkov, Victor V. ^{[11
]}

Vyalikh, Denis ^{[12
]}

Hunoldt, Sebastian ^{[3
]}

Woerheide, Gert ^{[13
,14
,15
]}

机构：

[1] TU Bergakad Freiberg, Inst Expt Phys, D-09599 Freiberg, Germany

[2] Limnol Inst SB RAS, Irkutsk 664033, Russia

[3] Tech Univ Dresden, Inst Bioanalyt Chem, D-01069 Dresden, Germany

[4] Leibniz Inst Polymer Res Dresden, D-01062 Dresden, Germany

[5] Max Bergmann Ctr Biomat, D-01062 Dresden, Germany

[6] Aix Marseille Univ, IRD, CNRS, Mediterranean Inst Biodivers & Ecol, F-13007 Marseille, France

[7] Tel Aviv Univ, Dept Zool, IL-69978 Tel Aviv, Israel

[8] Russian Acad Sci, PP Shirshov Oceanol Inst, Moscow, Russia

[9] Tech Univ Dresden, Inst Mat Sci, D-01062 Dresden, Germany

[10] Tech Univ Dresden, Carl Gustav Carus Klinikum, D-01069 Dresden, Germany

[11] Dept Math Komi SC UrD RAS, Syktyvkar, Russia

[12] Tech Univ Dresden, Inst Solid State Phys, D-01062 Dresden, Germany

[13] Univ Munich, Dept Earth & Environm Sci Palaeontol & Geobiol, D-80333 Munich, Germany

[14] Univ Munich, GeoBioctr, D-80333 Munich, Germany

[15] Bayer Staatssammlungen Palaontol & Geol, D-80333 Munich, Germany

来源：

JOURNAL OF STRUCTURAL BIOLOGY | 2013年 / 183卷 / 03期

关键词：

Sponges; Skeleton; Chitin; Chitin synthase; Bioanalytics; MARINE SPONGES; GLASS SPONGES; ALPHA-CHITIN; EVOLUTION; PORIFERA; PROTEIN; PART; EXPRESSION; BIOSILICA; SYNTHASE;

D O I：

10.1016/j.jsb.2013.06.015

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

This work demonstrates that chitin is an important structural component within the skeletal fibers of the freshwater sponge Spongilla lacustris. Using a variety of analytical techniques (C-13 solid state NMR, FT-IR, Raman, NEXAFS, ESI-MS, Morgan-Elson assay and Calcofluor White Staining); we show that this sponge chitin is much closer to alpha-chitin, known to be present in other animals, than to beta-chitin. Genetic analysis confirmed the presence of chitin synthases, which are described for the first time in a sponge. The presence of chitin in both marine (demosponges and hexactinellids) and freshwater sponges indicates that this important structural biopolymer was already present in their common ancestor. (C) 2013 Elsevier Inc. All rights reserved.

引用

页码：474 / 483

页数：10

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