Ultrasensitive and accelerated detection of ciguatoxin by capillary electrophoresis via on-line sandwich immunoassay with rotating magnetic field and nanoparticles signal enhancement

被引：15

作者：

Zhang, Zhaoxiang ^{[1
]}

Zhang, Chaoying ^{[1
]}

Luan, Wenxiu ^{[1
]}

Li, Xiufeng ^{[2
]}

Liu, Ying ^{[1
]}

Luo, Xiliang ^{[1
]}

机构：

[1] Qingdao Univ Sci & Technol, Coll Chem & Mol Engn, Key Lab Sensor Anal Tumor Marker, Minist Educ, Qingdao 266042, Peoples R China

[2] Shandong Hongjitang Pharmaceut Grp Co Ltd, Jinan 250103, Peoples R China

来源：

ANALYTICA CHIMICA ACTA | 2015年 / 888卷

基金：

中国国家自然科学基金;

关键词：

Nanoparticle; Sandwich immunoassay; Capillary electrophoresis; Electrochemical detection; Ciguatoxin; GOLD NANOPARTICLES; ELECTROCHEMICAL IMMUNOASSAY; ENZYME-IMMUNOASSAY; FISH; AMPLIFICATION; QUANTIFICATION; EXTRACTION; BIOMARKER; PROTEINS; DYNAMICS;

D O I：

10.1016/j.aca.2015.06.018

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A sensitive and rapid on-line immunoassay for the determination of ciguatoxin CTX3C was developed based on a capillary mixing system, which was integrated with capillary electrophoresis (CE) separation and electrochemical (EC) detection. In the sandwich immunoassay system, anti-CTX3C-functionalized magnetic nanoparticles were used as immunosensing probes, and horseradish peroxidase (HRP) and anti-CTX3C antibody were bound onto the surface of gold nanoparticles (AuNPs) and used as recognition elements. Online formation of immunocomplex was realized in capillary inlet end with an external rotating magnetic field. Compared with classical HPLC-MS and ELISA, the assay adopting AuNPs as multienzyme carriers and online sandwich immunoassay format with rotating magnetic field exhibited higher sensitivity and shorter assay time. The linear range of the assay for CTX3C was from 0.6 to 150 ng/L with a correlation coefficient of 0.9948 (n = 2), and the detection limit (S/N = 3) was 0.09 ng/L. The developed assay showed satisfying reproducibility and stability, and it was successfully applied for the quantification of CTX3C in fish samples. (C) 2015 Elsevier B.V. All rights reserved.

引用

页码：27 / 35

页数：9

共 38 条

[1] Enhanced Gold Nanoparticle Based ELISA for a Breast Cancer Biomarker [J].

Ambrosi, Adriano ;

Airo, Federico ;

Merkoci, Arben .

ANALYTICAL CHEMISTRY, 2010, 82 (03) :1151-1156

[2] Dispersive micro-solid-phase extraction of benzodiazepines from biological fluids based on polyaniline/magnetic nanoparticles composite [J].

Asgharinezhad, Ali Akbar ;

Ebrahimzadeh, Homeira ;

Mirbabaei, Fatemeh ;

Mollazadeh, Narges ;

Shekari, Nafiseh .

ANALYTICA CHIMICA ACTA, 2014, 844 :80-89

[3] Gold Nanoparticle Extraction Followed by Capillary Electrophoresis to Determine the Total, Free, and Protein-Bound Aminothiols in Plasma [J].

Chang, Chung-Wei ;

Tseng, Wei-Lung .

ANALYTICAL CHEMISTRY, 2010, 82 (07) :2696-2702

[4] Horseradish peroxidase-functionalized gold nanoparticle label for amplified immunoanalysis based on gold nanoparticles/carbon nanotubes hybrids modified biosensor [J].

Cui, Rongjing ;

Huang, Haiping ;

Yin, Zhengzhi ;

Gao, Di ;

Zhu, Jun-Jie .

BIOSENSORS & BIOELECTRONICS, 2008, 23 (11) :1666-1673

[5] Magnetic bead manipulation in a sub-microliter fluid volume applicable for biosensing [J].

Derks, R. J. S. ;

Dietzel, A. ;

Wimberger-Friedl, R. ;

Prins, M. W. J. .

MICROFLUIDICS AND NANOFLUIDICS, 2007, 3 (02) :141-149

[6] Magneto-mechanical mixing and manipulation of picoliter volumes in vesicles [J].

Franke, Thomas ;

Schmid, Lothar ;

Weitz, David A. ;

Wixforth, Achim .

LAB ON A CHIP, 2009, 9 (19) :2831-2835

[7] Stimuli-responsive controlled-release delivery system based on mesoporous silica nanorods capped with magnetic nanoparticles [J].

Giri, S ;

Trewyn, BG ;

Stellmaker, MP ;

Lin, VSY .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2005, 44 (32) :5038-5044

[8] What controls the melting properties of DNA-linked gold nanoparticle assemblies? [J].

Jin, RC ;

Wu, GS ;

Li, Z ;

Mirkin, CA ;

Schatz, GC .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2003, 125 (06) :1643-1654

[9] Rapid label-free visual assay for the detection and quantification of viral RNA using peptide nucleic acid (PNA) and gold nanoparticles (AuNPs) [J].

Joshi, Vinay G. ;

Chindera, Kantaraja ;

Singh, Arvind Kumar ;

Sahoo, Aditya P. ;

Dighe, Vikas D. ;

Thakuria, Dimpal ;

Tiwari, Ashok K. ;

Kumar, Satish .

ANALYTICA CHIMICA ACTA, 2013, 795 :1-7

[10] Ultrasensitive Multiplexed Immunoassay with Electrochemical Stripping Analysis of Silver Nanoparticles Catalytically Deposited by Gold Nanoparticles and Enzymatic Reaction [J].

Lai, Guosong ;

Yan, Feng ;

Wu, Jie ;

Leng, Chuan ;

Ju, Huangxian .

ANALYTICAL CHEMISTRY, 2011, 83 (07) :2726-2732

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