Compatibility of plant protein extraction methods with mass spectrometry for proteome analysis

被引：77

作者：

Sheoran, Inder S. ^{[1
]}

Ross, Andrew R. S. ^{[2
]}

Olson, Douglas J. H. ^{[2
]}

Sawhney, Vipen K. ^{[1
]}

机构：

[1] Univ Saskatchewan, Dept Biol, Saskatoon, SK S7N 5E2, Canada

[2] Natl Res Council Canada, Inst Plant Biotechnol, Saskatoon, SK S7N 0W9, Canada

来源：

PLANT SCIENCE | 2009年 / 176卷 / 01期

基金：

加拿大自然科学与工程研究理事会;

关键词：

Protein extraction methods; Mass Spectrometry; Pollen; Proteomics; 2-DIMENSIONAL GEL-ELECTROPHORESIS; MATURE POLLEN; TISSUES; ARABIDOPSIS; 2-DE; SOLUBILIZATION; PROTOCOL;

D O I：

10.1016/j.plantsci.2008.09.015

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Different protein extraction methods have been developed for plant proteome analysis but their compatibility with mass spectrometry has rarely been tested. We evaluated four protein extraction methods, i.e., trichloroacetic acid (TCA)-acetone, phenol, direct iso-electric focusing (IEF) buffer, and Tris-HCl buffer, using tomato pollen for proteome analysis. The data presented show that the TCA-acetone and phenol protein extraction methods are superior to the other two tested methods for tomato pollen proteome analysis, in terms of two-dimensional gel electrophoresis (2-DE) gel separation, mass spectrometric analysis, and identification of proteins by peptide mass fingerprinting (PMF). These results highlight the importance of plant protein extraction method for subsequent MS analysis and protein identification. (C) 2008 Elsevier Ireland Ltd. All rights reserved.

引用

页码：99 / 104

页数：6

共 27 条

[1] Effect of impurities on the matrix-assisted laser desorption/ionization mass spectra of insulin [J].

Bajuk, A ;

Gluch, K ;

Michalak, L .

RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 2001, 15 (24) :2383-2386

[2] Quantitative protein profiling using antibody arrays [J].

Barry, R ;

Soloviev, M .

PROTEOMICS, 2004, 4 (12) :3717-3726

[3] Preparation of protein extracts from recalcitrant plant tissues: An evaluation of different methods for two-dimensional gel electrophoresis analysis [J].

Carpentier, SC ;

Witters, E ;

Laukens, K ;

Deckers, P ;

Swennen, R ;

Panis, B .

PROTEOMICS, 2005, 5 (10) :2497-2507

[4] Calreticulin and calnexin in plants [J].

Crofts, AJ ;

Denecke, J .

TRENDS IN PLANT SCIENCE, 1998, 3 (10) :396-399

[5] TECHNICAL IMPROVEMENTS IN TWO-DIMENSIONAL ELECTROPHORESIS INCREASE THE LEVEL OF GENETIC-VARIATION DETECTED IN WHEAT-SEEDLING PROTEINS [J].

DAMERVAL, C ;

DEVIENNE, D ;

ZIVY, M ;

THIELLEMENT, H .

ELECTROPHORESIS, 1986, 7 (01) :52-54

[6] Potato tuber proteomics: Comparison of two complementary extraction methods designed for 2-DE of acidic proteins [J].

Delaplace, Pierre ;

van der Wal, Froukje ;

Dierick, Jean-Francois ;

Cordewener, Jan H. G. ;

Fauconnier, Marie-Laure ;

du Jardin, Patrick ;

America, Antoine H. R. .

PROTEOMICS, 2006, 6 (24) :6494-6497

[7] Differential proteomic analysis of four near-isogenic Brassica napus varieties bred for their erucic acid and glucosinolate contents [J].

Devouge, Vanessa ;

Rogniaux, Helene ;

Nesi, Nathalie ;

Tessier, Dominique ;

Gueguen, Jacques ;

Larre, Colette .

JOURNAL OF PROTEOME RESEARCH, 2007, 6 (04) :1342-1353

[8] Alternative and effective proteomic anaiysis in Arabidopsis [J].

Espagne, Christelle ;

Martinez, Aude ;

Valot, Benoit ;

Meinnel, Thierry ;

Giglione, Carmela .

PROTEOMICS, 2007, 7 (20) :3788-3799

[9] Proteomic analysis of Arabidopsis seed germination and priming [J].

Gallardo, K ;

Job, C ;

Groot, SPC ;

Puype, M ;

Demol, H ;

Vandekerckhove, J ;

Job, D .

PLANT PHYSIOLOGY, 2001, 126 (02) :835-848

[10] Extraction of proteins from plant tissues for two-dimensional electrophoresis analysis [J].

Giavalisco, P ;

Nordhoff, E ;

Lehrach, H ;

Gobom, J ;

Klose, J .

ELECTROPHORESIS, 2003, 24 (1-2) :207-216

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